Targeted gene therapy of LS174 T human colon carcinoma by anti-TAG-72 immunoliposomes

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dc.contributor.authorK S Kim-
dc.contributor.authorY K Lee-
dc.contributor.authorJ S Kim-
dc.contributor.authorK H Koo-
dc.contributor.authorHyo Jeong Hong-
dc.contributor.authorY S Park-
dc.date.accessioned2017-04-19T09:09:58Z-
dc.date.available2017-04-19T09:09:58Z-
dc.date.issued2008-
dc.identifier.issn0929-1903-
dc.identifier.uri10.1038/cgt.2008.11ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8382-
dc.description.abstractAnti-tumor-associated glycoprotein (TAG)-72 PEG-immunoliposomes (PILs) were prepared by conjugation of Fab′ fragments of recombinant humanized monoclonal antibody, HuCC49, to sterically stabilize unilamellar liposomes (90-110 nm in diameter) to target TAG-72-overexpressing cancer cells. The liposomes consisted of 1-palmitonyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC), 92 mol percent, O,O′-dymyrisyl-N-lysyl aspartate (DMKD cationic lipid), 4 mol percent, distearoyl-phosphatidyl-ethanolamine-polyethylene glycol 2000 (DSPE-PEG2000), 3 mol percent and DSPE-maleimide (DSPE-PEG 2000-Mal), 1 mol percent. These anti-TAG-72 PILs were able to adhere to the surface of TAG-72-overexpressing LS174 T human colon cancer cells more effectively than conventional liposomes. Also, in vitro gene transfection of the LS174 T cells by the anti-TAG-72 PILs in the presence of a high concentration of fetal bovine serum (up to 60%) was greater than that by conventional cationic lipoplexes. Intravenously administered anti-TAG-72 PILs efficiently localized in the LS174 T tumor tissues, while the non-targeted conventional liposomes did not. Intravenous administration of the anti-TAG-72 PILs containing plasmids encoding antiangiogenic proteins, such as angiostatin K1/3, endostatin and saxatilin, significantly inhibited in vivo growth of LS174 T tumors and angiogenesis in the tumor tissues. These results demonstrated the potential of TAG-72-mediated targeting of immunoliposomes as a modality for systemic gene delivery to human colon cancer cells.-
dc.publisherSpringer-Nature Pub Group-
dc.titleTargeted gene therapy of LS174 T human colon carcinoma by anti-TAG-72 immunoliposomes-
dc.title.alternativeTargeted gene therapy of LS174 T human colon carcinoma by anti-TAG-72 immunoliposomes-
dc.typeArticle-
dc.citation.titleCancer Gene Therapy-
dc.citation.number5-
dc.citation.endPage340-
dc.citation.startPage331-
dc.citation.volume15-
dc.contributor.affiliatedAuthorHyo Jeong Hong-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName홍효정-
dc.contributor.alternativeName-
dc.identifier.bibliographicCitationCancer Gene Therapy, vol. 15, no. 5, pp. 331-340-
dc.identifier.doi10.1038/cgt.2008.11-
dc.subject.keywordhumanized monoclonal antibody-
dc.subject.keywordPEG-immunoliposomes-
dc.subject.keywordTAG-72-
dc.subject.keywordtargeted gene therapy-
dc.subject.localhumanized monoclonal antibody-
dc.subject.localPEG-immunoliposomes-
dc.subject.localTAG-72-
dc.subject.localtargeted gene therapy-
dc.description.journalClassY-
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