DC Field | Value | Language |
---|---|---|
dc.contributor.author | K S Kim | - |
dc.contributor.author | Y K Lee | - |
dc.contributor.author | J S Kim | - |
dc.contributor.author | K H Koo | - |
dc.contributor.author | Hyo Jeong Hong | - |
dc.contributor.author | Y S Park | - |
dc.date.accessioned | 2017-04-19T09:09:58Z | - |
dc.date.available | 2017-04-19T09:09:58Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0929-1903 | - |
dc.identifier.uri | 10.1038/cgt.2008.11 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8382 | - |
dc.description.abstract | Anti-tumor-associated glycoprotein (TAG)-72 PEG-immunoliposomes (PILs) were prepared by conjugation of Fab′ fragments of recombinant humanized monoclonal antibody, HuCC49, to sterically stabilize unilamellar liposomes (90-110 nm in diameter) to target TAG-72-overexpressing cancer cells. The liposomes consisted of 1-palmitonyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC), 92 mol percent, O,O′-dymyrisyl-N-lysyl aspartate (DMKD cationic lipid), 4 mol percent, distearoyl-phosphatidyl-ethanolamine-polyethylene glycol 2000 (DSPE-PEG2000), 3 mol percent and DSPE-maleimide (DSPE-PEG 2000-Mal), 1 mol percent. These anti-TAG-72 PILs were able to adhere to the surface of TAG-72-overexpressing LS174 T human colon cancer cells more effectively than conventional liposomes. Also, in vitro gene transfection of the LS174 T cells by the anti-TAG-72 PILs in the presence of a high concentration of fetal bovine serum (up to 60%) was greater than that by conventional cationic lipoplexes. Intravenously administered anti-TAG-72 PILs efficiently localized in the LS174 T tumor tissues, while the non-targeted conventional liposomes did not. Intravenous administration of the anti-TAG-72 PILs containing plasmids encoding antiangiogenic proteins, such as angiostatin K1/3, endostatin and saxatilin, significantly inhibited in vivo growth of LS174 T tumors and angiogenesis in the tumor tissues. These results demonstrated the potential of TAG-72-mediated targeting of immunoliposomes as a modality for systemic gene delivery to human colon cancer cells. | - |
dc.publisher | Springer-Nature Pub Group | - |
dc.title | Targeted gene therapy of LS174 T human colon carcinoma by anti-TAG-72 immunoliposomes | - |
dc.title.alternative | Targeted gene therapy of LS174 T human colon carcinoma by anti-TAG-72 immunoliposomes | - |
dc.type | Article | - |
dc.citation.title | Cancer Gene Therapy | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 340 | - |
dc.citation.startPage | 331 | - |
dc.citation.volume | 15 | - |
dc.contributor.affiliatedAuthor | Hyo Jeong Hong | - |
dc.contributor.alternativeName | 김 | - |
dc.contributor.alternativeName | 이 | - |
dc.contributor.alternativeName | 김 | - |
dc.contributor.alternativeName | 구 | - |
dc.contributor.alternativeName | 홍효정 | - |
dc.contributor.alternativeName | 박 | - |
dc.identifier.bibliographicCitation | Cancer Gene Therapy, vol. 15, no. 5, pp. 331-340 | - |
dc.identifier.doi | 10.1038/cgt.2008.11 | - |
dc.subject.keyword | humanized monoclonal antibody | - |
dc.subject.keyword | PEG-immunoliposomes | - |
dc.subject.keyword | TAG-72 | - |
dc.subject.keyword | targeted gene therapy | - |
dc.subject.local | humanized monoclonal antibody | - |
dc.subject.local | PEG-immunoliposomes | - |
dc.subject.local | TAG-72 | - |
dc.subject.local | targeted gene therapy | - |
dc.description.journalClass | Y | - |
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