DC Field | Value | Language |
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dc.contributor.author | Tae-Hoon Kang | - |
dc.contributor.author | E I Hwang | - |
dc.contributor.author | Bong Sik Yun | - |
dc.contributor.author | C S Shin | - |
dc.contributor.author | Sung Uk Kim | - |
dc.date.accessioned | 2017-04-19T09:09:59Z | - |
dc.date.available | 2017-04-19T09:09:59Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.uri | 10.1248/bpb.31.755 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8388 | - |
dc.description.abstract | In the course of search for potent chitin synthase inhibitors from plant extracts, the chitin synthase 2 inhibitors, O-methyl pisiferic acid and 8,20-dihydroxy-9(11),13-abietadien-12-one which have diterpene skeleton, were isolated from the leaves of Chamaecyparis pisifera. These compounds inhibited chitin synthase 2 of Saccharomyces cerevisiae with the IC50 values of 5.8 and 226.4 μM, respectively. Especially, O-methyl pisiferic acid showed 15.3-fold stronger inhibitory activity than polyoxin D (IC50=88.6 μM), a well-known chitin synthase inhibitor. These compounds exhibited weaker inhibitory activities against chitin synthase 1 than chitin synthase 2, whereas it showed no inhibitory activity for chitin synthase 3. The compound exhibited mixed competitive inhibition with respect to UDP-N-acetyl-D-glucosamine as substrate (Ki=5 μM). These results indicated that O-methyl pisiferic acid is a specific inhibitor of chitin synthase 2. The compound also inhibited chitin synthase 1 of Candida albicans, which represents analogues to chitin synthase 2 of S. cerevisiae, with an IC50 of 75.6 μM, which represents 1.8-fold weaker activity than that of polyoxin D. Although O-methyl pisiferic acid has been reported for antibacterial and insecticidal activities, the present study is the first report on its inhibitory activity against chitin synthase 2. | - |
dc.publisher | Pharmaceutical Soc Japan | - |
dc.title | Chitin synthase 2 inhibitory activity of 0-methyl pisiferic acid and 8,20-dihydroxy-9(11),13-abietadien-12-one, isolated from Chamaecyparis pisifera = 화백으로부터 분리한 0-methyl pisiferic acid와 8,20-dihydroxy-9(11),13-abietadien-12-one 화합물의 키틴 | - |
dc.title.alternative | Chitin synthase 2 inhibitory activity of 0-methyl pisiferic acid and 8,20-dihydroxy-9(11),13-abietadien-12-one, isolated from Chamaecyparis pisifera | - |
dc.type | Article | - |
dc.citation.title | Biological & Pharmaceutical Bulletin | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 759 | - |
dc.citation.startPage | 755 | - |
dc.citation.volume | 31 | - |
dc.contributor.affiliatedAuthor | Tae-Hoon Kang | - |
dc.contributor.affiliatedAuthor | Bong Sik Yun | - |
dc.contributor.affiliatedAuthor | Sung Uk Kim | - |
dc.contributor.alternativeName | 강태훈 | - |
dc.contributor.alternativeName | 황의일 | - |
dc.contributor.alternativeName | 윤봉식 | - |
dc.contributor.alternativeName | 신철수 | - |
dc.contributor.alternativeName | 김성욱 | - |
dc.identifier.bibliographicCitation | Biological & Pharmaceutical Bulletin, vol. 31, no. 4, pp. 755-759 | - |
dc.identifier.doi | 10.1248/bpb.31.755 | - |
dc.subject.keyword | Antifungal activity | - |
dc.subject.keyword | Chamaecyparis pisifera | - |
dc.subject.keyword | Chitin synthase inhibitor | - |
dc.subject.keyword | O-methyl pisiferic acid | - |
dc.subject.local | Antifungal activity | - |
dc.subject.local | Anti-fungal activity | - |
dc.subject.local | antifungal activity | - |
dc.subject.local | Chamaecyparis pisifera | - |
dc.subject.local | Chitin synthase inhibitor | - |
dc.subject.local | O-methyl pisiferic acid | - |
dc.description.journalClass | Y | - |
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