DC Field | Value | Language |
---|---|---|
dc.contributor.author | S D Kim | - |
dc.contributor.author | In Kyoung Lee | - |
dc.contributor.author | W M Lee | - |
dc.contributor.author | J Y Cho | - |
dc.contributor.author | H J Park | - |
dc.contributor.author | J W Oh | - |
dc.contributor.author | S C Park | - |
dc.contributor.author | S K Kim | - |
dc.contributor.author | Y S Kwak | - |
dc.contributor.author | Bong Sik Yun | - |
dc.contributor.author | M H Rhee | - |
dc.date.accessioned | 2017-04-19T09:10:02Z | - |
dc.date.available | 2017-04-19T09:10:02Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0014-2999 | - |
dc.identifier.uri | 10.1016/j.ejphar.2008.02.008 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8403 | - |
dc.description.abstract | This study was designed to investigate the effect of davallialactone, which was isolated from the mushroom Inonotus xeranticus, on platelet aggregation induced by collagen, thrombin and ADP. We found that davallialactone dose-dependently inhibited platelet aggregation that was stimulated either by collagen (2.5 μg/ml), a potent ligand of integrin α2β1 and glycoprotein VI, or by thrombin (0.1U/ml), a potent agonist of the protease-activated receptors (PARs) PAR1 and PAR3. In addition, davallialactone inhibited platelet aggregation induced by ADP, an agonist of P2Y receptor. To understand the mechanism of anti-platelet activity, we determined whether davallialactone affected the downstream signaling in collagen-activated platelets. Using the fura-2/AM fluorometric assay, we found that davallialactone dose-dependently inhibited intracellular calcium concentration levels ([Ca2+]i). Moreover, davallialactone inhibited the phosphorylation of extracellular signal-regulated protein kinase (ERK)-2 and p38 mitogen-activated protein kinase (MAPK), in a dose-dependent manner. The tyrosine phosphorylation of 60 and 85kDa proteins, which were activated by collagen, were differentially inhibited by davallialactone. Taken together, these data suggest that davallialactone may have potential anti-platelet aggregation activity via suppression of intracellular downstream signaling pathways. | - |
dc.publisher | Elsevier | - |
dc.title | The mechanism of anti-platelet activity of davallialactone: Involvement of intracellular calcium ions, extracellular signal-regulated kinase 2 and p38 mitogen-activated protein kinase | - |
dc.title.alternative | The mechanism of anti-platelet activity of davallialactone: Involvement of intracellular calcium ions, extracellular signal-regulated kinase 2 and p38 mitogen-activated protein kinase | - |
dc.type | Article | - |
dc.citation.title | European Journal of Pharmacology | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 367 | - |
dc.citation.startPage | 361 | - |
dc.citation.volume | 584 | - |
dc.contributor.affiliatedAuthor | In Kyoung Lee | - |
dc.contributor.affiliatedAuthor | Bong Sik Yun | - |
dc.contributor.alternativeName | 김성대 | - |
dc.contributor.alternativeName | 이인경 | - |
dc.contributor.alternativeName | 이희민 | - |
dc.contributor.alternativeName | 조재열 | - |
dc.contributor.alternativeName | 박화진 | - |
dc.contributor.alternativeName | 오재욱 | - |
dc.contributor.alternativeName | 박승춘 | - |
dc.contributor.alternativeName | 김상근 | - |
dc.contributor.alternativeName | 곽이성 | - |
dc.contributor.alternativeName | 윤봉식 | - |
dc.contributor.alternativeName | 이만희 | - |
dc.identifier.bibliographicCitation | European Journal of Pharmacology, vol. 584, no. 2, pp. 361-367 | - |
dc.identifier.doi | 10.1016/j.ejphar.2008.02.008 | - |
dc.subject.keyword | [Ca2+]i | - |
dc.subject.keyword | ADP | - |
dc.subject.keyword | collagen | - |
dc.subject.keyword | davallialactone | - |
dc.subject.keyword | ERK2 | - |
dc.subject.keyword | inonotus xeranticus | - |
dc.subject.keyword | p38 MAPK | - |
dc.subject.keyword | platelet aggregation | - |
dc.subject.keyword | thrombin | - |
dc.subject.local | [Ca2+]i | - |
dc.subject.local | ADP | - |
dc.subject.local | Collagen | - |
dc.subject.local | collagen | - |
dc.subject.local | Davallialactone | - |
dc.subject.local | davallialactone | - |
dc.subject.local | ERK2 | - |
dc.subject.local | inonotus xeranticus | - |
dc.subject.local | Inonotus xeranticus | - |
dc.subject.local | p38 MAP kinase | - |
dc.subject.local | p38 mitogen-activated protein kinase (p38 MARK) | - |
dc.subject.local | P38 MAPK | - |
dc.subject.local | p38 mitogen-activated protein kinase | - |
dc.subject.local | p38 MAPK | - |
dc.subject.local | p38MAPK | - |
dc.subject.local | Platelet aggregation | - |
dc.subject.local | platelet aggregation | - |
dc.subject.local | thrombin | - |
dc.subject.local | Thrombin | - |
dc.description.journalClass | Y | - |
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