Quantum dot-based protein micro- and nanoarrays for detection of prostate cancer biomarkers

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Title
Quantum dot-based protein micro- and nanoarrays for detection of prostate cancer biomarkers
Author(s)
A Gokarna; L H Jin; J S Hwang; Y H Cho; Yong Taik Lim; Bong Hyun Chung; S H Youn; D S Choi; J H Lim
Bibliographic Citation
Proteomics, vol. 8, no. 9, pp. 1809-1818
Publication Year
2008
Abstract
In this article, we demonstrate the fabrication and detection of cancer protein biochips consisting of micro- and nanoarrays whereby pegylated quantum dots (QDs) conjugated to antibodies (Abs) of prostate specific antigens (PSA) were used for the detection of clinical biomarkers such as PSA. BSA which acts as an efficient blocking layer in microarrays, tends to show an interaction with QDs. In view of this fact, we investigated two series of samples which were fabricated in the presence and absence of BSA blocking layer. Variation in the incubation time required for the antigen-antibody interaction to take place, different proteins as controls and the effect of bare QDs on these microarrays, were the three main parameters which were studied in these two series. Samples fabricated in the absence of BSA blocking layer exhibited an extremely high specificity in the detection of cancer proteins and were also marked by negligible nonspecific binding effects of QDs, in stark contrast to the samples fabricated using BSA as a blocking layer. Fabrication of nanoarrays of QD-conjugated PSA Abs having a spot size of nearly 900 nm has also been demonstrated. Thus, we show the potential offered by QDs in in vitro analysis of cancer biomarker imaging.
Keyword
dip-pen nanolithographyprostate specific antigenprotein microarraysprotein nanoarraysquantum dots
ISSN
1615-9853
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/pmic.200701072
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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