Suppression of inducible nitric oxide synthase and cyclooxygenase-2 expression by tussilagone from farfarae flos in BV-2 microglial cells

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Title
Suppression of inducible nitric oxide synthase and cyclooxygenase-2 expression by tussilagone from farfarae flos in BV-2 microglial cells
Author(s)
H J Lim; Hyun Sun Lee; J H Ryu
Bibliographic Citation
Archives of Pharmacal Research, vol. 31, no. 5, pp. 645-652
Publication Year
2008
Abstract
Activated microglia produce diverse neurotoxic factors such as nitric oxide (NO) and prostaglandin E2 (PGE2) that may cause neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. From the EtOAc soluble fraction of Farfarae flos (Tussilago farfara), we purified tussilagone as a bioactive compound by monitoring the inhibitory potential of NO production in activated microglia through the purification procedures. Tussilagone showed dose-dependent inhibition of NO and PGE 2 production in LPS-activated microglia with IC50 values of 8.67 μM and 14.1 μM, respectively. It suppressed the expression of protein and mRNA of inducible nitric oxide synthase and cyclooxygenase-2 through the inhibition of 1-κBα degradation and nuclear translocation of p65 subunit of NF-κB. Therefore tussilagone from Farfarae flos may have therapeutic potential in the treatment of neuro-inflammatory diseases through the inhibition of overproduction of NO and PGE2.
Keyword
cyclooxygenasefarfarae flosinhibitor kappaB-αnitric oxidenitric oxide synthasePGE 2tussilagone
ISSN
0253-6269
Publisher
Pharmaceutical Soc Korea
DOI
http://dx.doi.org/10.1007/s12272-001-1207-4
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
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