Transcriptional regulation of GSDML gene by antisense-oriented HERV-H LTR element = Antisense바향의 HERV-H LTR로 인한 GSDML유전자의 전사조절 기작 연구

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Title
Transcriptional regulation of GSDML gene by antisense-oriented HERV-H LTR element = Antisense바향의 HERV-H LTR로 인한 GSDML유전자의 전사조절 기작 연구
Author(s)
Jae Won Huh; D S Kim; D W Kang; H S Ha; K Ahn; Y N Noh; D S Min; Kyu Tae Chang; H S Kim
Bibliographic Citation
Archives of Virology, vol. 153, no. 6, pp. 1201-1205
Publication Year
2008
Abstract
During the course of hominoid evolution, a new transcript variant of the GSDML (gasdermin-like protein) gene was formed by the integration of the antisense-oriented HERV-H (human endogenous retrovirus) LTR (long terminal repeat) element. To investigate regions that are critical for transcriptional regulation of the GSDML gene, we generated seven deletion mutants from a full-length clone (clone 1/630) that includes the HERV-H LTR sequence and compared their expression levels relative to the full-length parental clone using a transient transfection assay. In the transient transfection assay, deletion of the 5′ flanking region (cellular origin) of the HERV-H LTR sequence led to a 4.5-fold increase in expression compared to the full-length clone, while deletion of the U5 region showed a significant decrease in transcriptional activity. Deletion of the 3′ flanking region of the LTR sequence (clone 42/451) showed similar transcriptional activity to a clone missing the 5′ flanking region of cellular origin (clone 42/630). Taken together, these data indicate that the HERV-H LTR sequence (viral origin) positively regulates transcriptional activity of the GSDML gene and that the 5′ flanking region sequence (cellular origin) exerts negative transcriptional regulation.
ISSN
0304-8608
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00705-008-0105-y
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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