Ultra-fast simultaneous detection of obesity-related coenzymes in mice using microchip electrophoresis with a LIF detector

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dc.contributor.authorHee Gu Lee-
dc.contributor.authorK S Kumar-
dc.contributor.authorJ R Soh-
dc.contributor.authorY S Cha-
dc.contributor.authorS H Kang-
dc.date.accessioned2017-04-19T09:10:16Z-
dc.date.available2017-04-19T09:10:16Z-
dc.date.issued2008-
dc.identifier.issn0003-2670-
dc.identifier.uri10.1016/j.aca.2008.01.012ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8435-
dc.description.abstractHepatic acyl-coenzyme A synthetase (ACS), carnitine palmitoyltransferase-I (CPT-I) and acetyl coenzyme A carboxylase (ACC) are coenzymes associated with the genetic type of obesity in animal models. This paper reports the use of microchip electrophoresis (ME) with a laser-induced fluorescence (LIF) detector based on a reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the amplified DNA fragments of these coenzymes (ACS, CPT-I and ACC) in the mRNA extracted from mice. DNA fragments ranging from 50 to 2652 bp were well resolved using this procedure with a running buffer (1× TBE), 0.5% polyvinylpyrrolidone (Mr 1,000,000) as the coating gel and 0.7% polyethyleneoxide (Mr 8,000,000) as the sieving gel at pH 8.30. The separation of the three RT-PCR products was achieved by ME in a single-run within 17 s using programmed field strength gradients (PFSG) (470 V cm-1 for 9 s, 205.8 V cm-1 for 2 s, 411.6 V cm-1 for 4 s, 117.6 V cm-1 for 2 s and 470.4 V cm-1 for 8 s). The ME-PFSG method was found to be 4 times faster than the method using a constant field and 138 times faster than slab gel electrophoresis. Moreover, the amplified RT-PCR products of the obesity-related coenzymes in C57BL/6J mice were analyzed using only sub-micro liter samples.-
dc.publisherElsevier-
dc.titleUltra-fast simultaneous detection of obesity-related coenzymes in mice using microchip electrophoresis with a LIF detector-
dc.title.alternativeUltra-fast simultaneous detection of obesity-related coenzymes in mice using microchip electrophoresis with a LIF detector-
dc.typeArticle-
dc.citation.titleAnalytica Chimica Acta-
dc.citation.number1-
dc.citation.endPage100-
dc.citation.startPage94-
dc.citation.volume619-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.alternativeName이희구-
dc.contributor.alternativeNameKumar-
dc.contributor.alternativeName서주련-
dc.contributor.alternativeName차윤수-
dc.contributor.alternativeName강성호-
dc.identifier.bibliographicCitationAnalytica Chimica Acta, vol. 619, no. 1, pp. 94-100-
dc.identifier.doi10.1016/j.aca.2008.01.012-
dc.subject.keywordcoenzymes-
dc.subject.keywordmicrochip electrophoresis-
dc.subject.keywordobesity-
dc.subject.keywordultra-fast detection-
dc.subject.localcoenzymes-
dc.subject.localmicrochip electrophoresis-
dc.subject.localObesity-
dc.subject.localobesity-
dc.subject.localultra-fast detection-
dc.description.journalClassY-
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Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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