Role of STAT3 as a negative regulator in Mac2-binding protein expression = Mac-2 결합단백질 발현에서의 STAT3의 역할

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dc.contributor.authorYuk Pheel Park-
dc.contributor.authorJong-Tae Kim-
dc.contributor.authorY Yang-
dc.contributor.authorJ S Lim-
dc.contributor.authorD Y Yoon-
dc.contributor.authorJ W Kim-
dc.contributor.authorHee Gu Lee-
dc.date.accessioned2017-04-19T09:10:17Z-
dc.date.available2017-04-19T09:10:17Z-
dc.date.issued2008-
dc.identifier.issnI000-0170-
dc.identifier.uri10.3343/kjlm.2008.28.3.230ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8436-
dc.description.abstractBACKGROUND: Mac-2 binding protein (Mac-2BP) is a secreted glycoprotein from the culture fluid of several human cancer cells, especially breast, lung, and gastric cells. Mac-2BP plays a role in immune response and cell adhesion activity in patients with various cancer and infectious diseases. In this study, we attempted to identify the regulators of Mac-2BP expression at the transcriptional level. METHODS: To determine the effect of epidermal growth factor (EGF) to Mac-2BP expression in gastric cancers, we constructed the different lengths of Mac-2BP promoter plasmids and measured the promoter activity and Mac-2BP expression. In addition to investigating the role of signal transducer and activator of transcription3 (STAT3) or human telomerase reverse transcriptase (hTERT) as a regulator of Mac-2BP, we transfected the small interfering RNA (siRNA) specific for STAT3 or hTERT, and Mac-2BP level was observed by a quantitative ELISA. RESULTS: EGF treatment could suppress the Mac-2BP transcription in HEK293 or gastric cancer cell lines (SNU-638 or AGS). In 5'-deleted promoter experiment, pGL3-Mac Pro-2377 transfected cells showed a decreased luciferase activity compared to pGL3-Mac Pro-2277. We also identified that (-2,366/-2,356) on Mac-2BP promoter is a putative STAT3 binding site and suppression of STAT3 with STAT3 specific siRNA increased the Mac-2BP level, suggesting the role of STAT3 as a negative regulator, in contrast to hTERT, which is known as a positive regulator. CONCLUSIONS: EGF signal is critical for the Mac-2BP expression, and more importantly, STAT3 could work as a negative regulator, while hTERT as a positive regulator in Mac-2BP transcription.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleRole of STAT3 as a negative regulator in Mac2-binding protein expression = Mac-2 결합단백질 발현에서의 STAT3의 역할-
dc.title.alternativeRole of STAT3 as a negative regulator in Mac2-binding protein expression-
dc.typeArticle-
dc.citation.titleKorean Journal of Laboratory Medicine-
dc.citation.number3-
dc.citation.endPage238-
dc.citation.startPage230-
dc.citation.volume28-
dc.contributor.affiliatedAuthorYuk Pheel Park-
dc.contributor.affiliatedAuthorJong-Tae Kim-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.alternativeName박육필-
dc.contributor.alternativeName김종태-
dc.contributor.alternativeName양영-
dc.contributor.alternativeName임종석-
dc.contributor.alternativeName윤도영-
dc.contributor.alternativeName김종완-
dc.contributor.alternativeName이희구-
dc.identifier.bibliographicCitationKorean Journal of Laboratory Medicine, vol. 28, no. 3, pp. 230-238-
dc.identifier.doi10.3343/kjlm.2008.28.3.230-
dc.subject.keywordEGF-
dc.subject.keywordhTERT-
dc.subject.keywordMac-2BP-
dc.subject.keywordSTAT3-
dc.subject.localEGF-
dc.subject.localhTERT-
dc.subject.localMac-2 BP-
dc.subject.localMac-2BP-
dc.subject.localSignal transducer and activator of transcription 3 (STAT3)-
dc.subject.localSignal transducer and activator of transcription-
dc.subject.localSignal transducer and activator of transcription 3 (Stat3)-
dc.subject.localSTAT 3-
dc.subject.localSTAT3-
dc.subject.localSignal transducer and activator of transcription 3-
dc.subject.localStat3-
dc.subject.localSignal transducer and activator of transcription factor 3 (STAT3)-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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