Effects of a tetramethoxyhydroxyflavone on the expression of inflammatory mediators in LPS-treated human synovial fibroblast and macrophage cells

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Title
Effects of a tetramethoxyhydroxyflavone on the expression of inflammatory mediators in LPS-treated human synovial fibroblast and macrophage cells
Author(s)
D Yoon; M Cho; J H Kim; E J Kim; J W Kang; E H Seo; J H Shim; S H Kim; Hee Gu Lee; G T Oh; J T Hong; J W Park; J W Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 18, no. 4, pp. 686-694
Publication Year
2008
Abstract
The inhibitory effects of 5,6,3′,5′-tetramethoxy 7,4′-hydroxyflavone (labeled as p7F) were elucidated on the productions of proinflammatory cytokines as well as inflammatory mediators in human synovial fibroblasts and macrophage cells. p7F inhibited IL-1β or TNF-α induced expressions of inflammatory mediators (ICAM-1, COX-2, and iNOS). p7F also inhibited LPS-induced productions of nitric oxide and prostaglandin E2 in RAW 264.7 cells. In order to investigate whether p7F would inhibit IL-1 signaling, p7F was added to the D10S Th2 cell line (which is responsive to only IL-1β and thus proliferates), revealing that p7F inhibited IL-1β-induced proliferation of D10S Th2 cells in a dose-response manner. A flow cytometric analysis revealed that p7F reduced the intracellular level of free radical oxygen species in RAW 264.7 cells treated with hydrogen peroxide. p7F inhibited IκB degradation and NF-κB activation in macrophage cells treated with LPS, supporting that p7F could inhibit signaling mediated via toll-like receptor. Taken together, p7F has inhibitory effects on LPS-induced productions of inflammatory mediators on human synovial fibroblasts and macrophage cells and thus has the potential to be an anti-inflammatory agent for inhibiting inflammatory responses.
Keyword
anti-inflammatory agentflavoricinflammatory mediatorsproinflammatory cytokinessynovial fibroblasts
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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