Elevated H2O2 production via overexpression of a chloroplastic Cu/ZnSOD gene of lily (Lilium oriental hybrid 'Marco Polo') triggers ethylene synthesis in transgenic potato

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Title
Elevated H2O2 production via overexpression of a chloroplastic Cu/ZnSOD gene of lily (Lilium oriental hybrid 'Marco Polo') triggers ethylene synthesis in transgenic potato
Author(s)
Yoon Sik Kim; Hyun Soon Kim; Yong Hwa Lee; Mi-Seon Kim; Hyun Woo Oh; K W Hahn; Hyouk Joung; Jae Heung Jeon
Bibliographic Citation
Plant Cell Reports, vol. 27, no. 6, pp. 973-983
Publication Year
2008
Abstract
Transgenic potato plants (SS2 and SS4) that overexpressed a chloroplastic copper/zinc superoxide dismutase lily gene were utilized as an H 2O2-inducible system in order to study the role of H 2O2 as a signaling molecule in the biosynthesis of ethylene. SS2 and SS4 plants grown in vitro under sealed microenvironment (SME) conditions displayed anomalous phenotypes including reduction of stem elongation, radial stem growth, and promotion of root hair formation in the generated root, which were similar to ethylene-induced responses. In addition, SS4 plants showed severe vitrification in developing leaves and elevated ethylene production under SME conditions. After the ethylene action inhibitor AgNO3, 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) inhibitor CoCl2, and ACC synthase inhibitor l-aminoethoxyvinylglycine were added to the growth media, the anomalous phenotypes in SS4 plants reverted to their normal phenotype with a concurrent decrease in ethylene production. Northern blot analysis showed that ACO transcripts in SS4 plants were constantly at high levels under normal and SME conditions, indicating that a high level of H2O2 in SS4 plants up-regulates ACO transcripts. Moreover, the direct treatment of H2O2 in potato plants confirmed the elevated expression of the ACO gene. Taken together, these data suggest that the high concentration of H2O2 in transgenic potato plants stimulates ethylene biosynthesis by activating ACO gene expression.
Keyword
VitrificationACOCu/Zn-superoxide dismutaseH2O2Transgenic potato
ISSN
0721-7714
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00299-008-0515-z
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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