dSETDB1 and SU(VAR)3-9 sequentially function during germline-stem cell differentiation in Drosophila melanogaster

Cited 53 time in scopus
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Title
dSETDB1 and SU(VAR)3-9 sequentially function during germline-stem cell differentiation in Drosophila melanogaster
Author(s)
Jeongheon Yoon; Kyu-Sun LeeJung Sun ParkKweon Yu; S G Paik; Yong-Kook Kang
Bibliographic Citation
PLoS One, vol. 3, no. 5, pp. e2234-e2234
Publication Year
2008
Abstract
Germline-stem cells (GSCs) produce garnetes and are thus true "immortal stem cells". In Drosophila ovaries, GSCs divide asymmetrically to produce daughter GSCs and cystoblasts, and the latter differentiate into germline cysts. Here we show that the histone-lysine methyltransferase dSETDB1, located in pericentric heterochromatin, catalyzes H3-K9 trimethylation in GSCs and their immediate descendants. As germline cysts differentiate into egg chambers, the dSETDB1 function is gradually taken over by another H3-K9-specific methyltransferase, SU(VAR)3-9. Loss of function mutations in dsetdb1 or Su(var)3-9 abolish both H3K9me3 and heterochromatin protein 1 (HP1) signals from the anterior germarium and the developing egg chambers, respectively, and cause localization of H3K9me3 away from DNA-dense regions in most posterior germarium cells. These results indicate that dSETDB1 and SU(VAR)3-9 act together with distinct roles during oogenesis, with dsetcb1 being of particular importance due to its GSC-specific function and more severe mutant phenotype.
ISSN
1932-6203
Publisher
Public Library of Science
DOI
http://dx.doi.org/10.1371/journal.pone.0002234
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > 1. Journal Articles
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