Biochemical charactrization of a glycosyltransferase homolog from an oral pathogen Fusobacterium nucleatum as a human glycan-modifying enzyme

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dc.contributor.authorSeong Hun Kim-
dc.contributor.authorDoo-Byoung Oh-
dc.contributor.authorOh Suk Kwon-
dc.contributor.authorJ K Jeong-
dc.contributor.authorYun Mi Lee-
dc.contributor.authorK Ko-
dc.contributor.authorJeong Heon Ko-
dc.contributor.authorH A Kang-
dc.date.accessioned2017-04-19T09:11:04Z-
dc.date.available2017-04-19T09:11:04Z-
dc.date.issued2008-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8486-
dc.description.abstractBacterial glycosyltransferases have drawn growing attention as economical enzymes for oligosaccharide synthesis, with their easy expression and relatively broad substrate specificity. Here, we characterized a glycosyltransferase homolog (Fnu_GT) from a human oral pathogen, Fusobacterium nucleatum. Bioinformatic analysis showed that Fnu_GT belongs to the glycosyltransferases family II. The recombinant Fnu_GT (rFnu_GT) expressed in Escherichia coli displayed the highest glycosylation activity when UDP-galactose (Gal) was used as a donor nucleotide-sugar with heptose or Nacetylglucosamine (GlcNAc) as an acceptor sugar. Interestingly, rFnu_GT transferred the galactose moiety of UDP-Gal to a nonreducing terminal GlcNAc attached to the trimannosyl core glycan, indicating its potential as an enzyme for humantype N-glycan synthesis-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleBiochemical charactrization of a glycosyltransferase homolog from an oral pathogen Fusobacterium nucleatum as a human glycan-modifying enzyme-
dc.title.alternativeBiochemical charactrization of a glycosyltransferase homolog from an oral pathogen Fusobacterium nucleatum as a human glycan-modifying enzyme-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number5-
dc.citation.endPage865-
dc.citation.startPage859-
dc.citation.volume18-
dc.contributor.affiliatedAuthorSeong Hun Kim-
dc.contributor.affiliatedAuthorDoo-Byoung Oh-
dc.contributor.affiliatedAuthorOh Suk Kwon-
dc.contributor.affiliatedAuthorYun Mi Lee-
dc.contributor.affiliatedAuthorJeong Heon Ko-
dc.contributor.alternativeName김성훈-
dc.contributor.alternativeName오두병-
dc.contributor.alternativeName권오석-
dc.contributor.alternativeName정재갑-
dc.contributor.alternativeName이윤미-
dc.contributor.alternativeName고기성-
dc.contributor.alternativeName고정헌-
dc.contributor.alternativeName강현아-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 18, no. 5, pp. 859-865-
dc.subject.keywordGlycosyltransferase-
dc.subject.keywordnucleotide-sugar-
dc.subject.keywordFusobacterium nucleatum-
dc.subject.keywordhuman-type N-linked glycan-
dc.subject.localGlycosyltransferase-
dc.subject.localglycosyltransferase-
dc.subject.localGlycosyltransferases-
dc.subject.localnucleotide-sugar-
dc.subject.localFusobacterium nucleatum-
dc.subject.localhuman-type N-linked glycan-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Aging Convergence Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
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