Enterococcus thailandicus sp. nov., isolated from fermented sausage ('mum') in Thailand

Abstract

A Gram-positiva and catalase-negative coccus that formed chains, designated strain FP48-3T, isolated from fermented sausage ('mum'), was studied systematically. Strain FP48-3T as facultatively anaerobic and produced L-lactic acid from glucose. Straight-chain fatty acids C18:1 and C16:0 were the dominant components. The DNA G + C content of strain FP48-3T was 37.9 mol%. On the basis of 16S rRNA and RNA polymerase α-subunit (rpoA) gene sequence analysis, strain FP48-3T was closely related to Enterococcus hirae LMG 6399T , Enterococcus durans LMG 10746T and Enterococcus faecium LMG 11423T, with 99.3-99.6 and 95.1-96.9 % sequence similarities, respectively. Strain FP48-3T could be clearly distinguished from E. hirae LMG 6399T, E. durans LMG 10746T and E. faecium LMG 11423T by low DNA-DNA relatedness (≤ 14 %) and phenotypic characteristics. Therefore, this strain represents a novel species of the genus Enterococcus, for which the name Enterococcus thailandicus sp. nov. is proposed. The type strain is FP48-3T (=KCTC 13134T = NBRC 101867T = NRIC 0107T = TISTR 933T = PCU 282T).