Transcriptional targeting of gene expression in breast cancer by the promoters of protein regulator of cytokinesis 1 and ribonuclease reductase 2 = 싸이토키네시스1 과 리보뉴클레아제 리덕테아제 2의 프로모터에 의한 유방암에서의 유전자 발현의 전사 표적

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dc.contributor.authorH J Yun-
dc.contributor.authorY H Cho-
dc.contributor.authorY Moon-
dc.contributor.authorYoung Woo Park-
dc.contributor.authorH K Yoon-
dc.contributor.authorY J Kim-
dc.contributor.authorS H Cho-
dc.contributor.authorY I Lee-
dc.contributor.authorB S Kang-
dc.contributor.authorW J Kim-
dc.contributor.authorK Park-
dc.contributor.authorW Seol-
dc.date.accessioned2017-04-19T09:11:08Z-
dc.date.available2017-04-19T09:11:08Z-
dc.date.issued2008-
dc.identifier.issnI000-0028-
dc.identifier.uri10.3858/emm.2008.40.3.345ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8491-
dc.description.abstractFor cancer gene therapy, cancer-specific overexpression of a therapeutic gene is required to reduce side effects derived from expression of the gene in normal cells. To develop such an expression vector, we searched for genes over-expressed and/or specifically expressed in cancer cells using bioinformatics and have selected genes coding for protein regulator of cytokinesis 1 (PRC1) and ribonuclease reductase 2 (RRM2) as candidates. Their cancer-specific expressions were confirmed in both breast cancer cell lines and patient tissues. We compared each promoter's cancer-specific activity in the breast normal and cancer cell lines using the luciferase gene as a reporter and confirmed cancer-specific expression of both PRC1 and RRM2 promoters. To test activities of these promoters in viral vectors, the promoters were also cloned into an adeno-associated viral (AAV) vector containing green fluorescence protein (GFP) as the reporter. The GFP expression levels by these promoters were various depending on cell lines tested and, in MDA-MB-231 cells, GFP activities derived from the PRC1 and RRM2 promoters were as strong as that from the cytomegalovirus (CMV) promoter. Our result showed that a vector containing the PRC1 or RRM2 promoter could be used for breast cancer specific overexpression in gene therapy.-
dc.publisherSpringer-Nature Pub Group-
dc.titleTranscriptional targeting of gene expression in breast cancer by the promoters of protein regulator of cytokinesis 1 and ribonuclease reductase 2 = 싸이토키네시스1 과 리보뉴클레아제 리덕테아제 2의 프로모터에 의한 유방암에서의 유전자 발현의 전사 표적-
dc.title.alternativeTranscriptional targeting of gene expression in breast cancer by the promoters of protein regulator of cytokinesis 1 and ribonuclease reductase 2-
dc.typeArticle-
dc.citation.titleExperimental and Molecular Medicine-
dc.citation.number3-
dc.citation.endPage353-
dc.citation.startPage345-
dc.citation.volume40-
dc.contributor.affiliatedAuthorYoung Woo Park-
dc.contributor.alternativeName윤혜진-
dc.contributor.alternativeName조영화-
dc.contributor.alternativeName문영선-
dc.contributor.alternativeName박영우-
dc.contributor.alternativeName윤혜경-
dc.contributor.alternativeName김연주-
dc.contributor.alternativeName조성하-
dc.contributor.alternativeName이영일-
dc.contributor.alternativeName강봉수-
dc.contributor.alternativeName김운재-
dc.contributor.alternativeName박기랑-
dc.contributor.alternativeName설원기-
dc.identifier.bibliographicCitationExperimental and Molecular Medicine, vol. 40, no. 3, pp. 345-353-
dc.identifier.doi10.3858/emm.2008.40.3.345-
dc.subject.keywordbreast neoplasms-
dc.subject.keywordgene therapy-
dc.subject.keywordPRC1 protein, human-
dc.subject.keywordribonucleotide reductase M2-
dc.subject.localbreast neoplasms-
dc.subject.localBreast neoplasms-
dc.subject.localGene Therapy-
dc.subject.localgene therapy-
dc.subject.localGene therapy-
dc.subject.localPRC1 protein, human-
dc.subject.localribonucleotide reductase M2-
dc.description.journalClassY-
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