Engineering hybrid proteins by modular recombination and evolutionary optimization = 모듈성 단백질의 재설계 및 개량

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Title
Engineering hybrid proteins by modular recombination and evolutionary optimization = 모듈성 단백질의 재설계 및 개량
Author(s)
Seung Goo LeeEugene Rha; Jae-Seok Ha; Jeong Min Lee; Sun-Hwa Kim
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 36, no. 2, pp. 149-157
Publication Year
2008
Abstract
Many proteins consist of distinctive domains that can act independently or cooperatively to achieve a unique function. As these domains evolve from a naturally existing repertoire of functional domains, this implies that domain organization is an intrinsic element involved in building the complex structure and function of proteins. Thus, identifying functional domains would appear to be critical to the elucidation of questions related to protein evolution, folding, and the engineering of hybrid proteins for tailored applications. However, the simple application of “Lego-like assembly” to the engineering of hybrid proteins is an oversimplification, as many hybrid constructs lack structural stability, usually due to unfavorable domain contacts. Thus, directed evolution, along with computational studies, may help to engineer hybrid proteins with improved physico-chemical properties. Accordingly, this paper introduces several approaches to functional hybrid protein engineering that potentially can be used to create modulators of gene transcription and cell signaling, and novel biosensors to analyze biological functions in vivo.
Keyword
modularityprotein engineeringdirected evolutiondomainredesign
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
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