|dc.contributor.author||K E Lee||-|
|dc.contributor.author||J K Lee||-|
|dc.contributor.author||Byoung Chul Park||-|
|dc.contributor.author||K S Kim||-|
|dc.description.abstract||The free-living photoheterotrophic Gram-negative bacterium Rhodobacter sphaeroides possesses a quorum-sensing (QS) regulatory system mediated by CerR-CerI, a member of the LuxR-LuxI family. To identify the genes affected by the regulatory system, random lacZ fusions were generated in the genome of R. sphaeroides strain 2.4.1 using a promoter-trapping vector, pSG2. About 20,000 clones were screened and 23 showed a significantly different level of β-gal activities upon the addition of synthetic 7,8-cis-N-tetradecenoyl-homoserine lactone (RAI). Among these 23 clones, the clone showing the highest level of induction was selected for further study, where about a ten-fold increase of β-gal activity was exhibited in the presence of RAI and induction was shown to be required for cerR. In this clone, the 1acZ reporter was inserted in a putative gene that exhibited a low homology with catD. A genetic analysis showed that the expression of the catD homolog was initiated from a promoter of another gene present upstream of the catD. This upstream gene showed a strong homology with luxR and hence was named qsrR (quorum-sensing regulation regulator). A comparison of the total protein expression profiles for the wild-type cells and qsrR-null mutant cells using two-dimensional gel electrophoresis and a MALDI-TOF analysis allowed the identification of sets of genes modulated by the luxR homolog.||-|
|dc.title||Genes of Rhodobacter sphaeroides 2.4.1 regulated by innate quorum-sensing signal, 7,8-cis-N-(tetradecenoyl) homoserine lactone||-|
|dc.title.alternative||Genes of Rhodobacter sphaeroides 2.4.1 regulated by innate quorum-sensing signal, 7,8-cis-N-(tetradecenoyl) homoserine lactone||-|
|dc.citation.title||Journal of Microbiology and Biotechnology||-|
|dc.contributor.affiliatedAuthor||Byoung Chul Park||-|
|dc.identifier.bibliographicCitation||Journal of Microbiology and Biotechnology, vol. 18, no. 2, pp. 219-227||-|
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