Genes of Rhodobacter sphaeroides 2.4.1 regulated by innate quorum-sensing signal, 7,8-cis-N-(tetradecenoyl) homoserine lactone

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dc.contributor.authorW Hwang-
dc.contributor.authorK E Lee-
dc.contributor.authorJ K Lee-
dc.contributor.authorByoung Chul Park-
dc.contributor.authorK S Kim-
dc.date.accessioned2017-04-19T09:11:10Z-
dc.date.available2017-04-19T09:11:10Z-
dc.date.issued2008-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8504-
dc.description.abstractThe free-living photoheterotrophic Gram-negative bacterium Rhodobacter sphaeroides possesses a quorum-sensing (QS) regulatory system mediated by CerR-CerI, a member of the LuxR-LuxI family. To identify the genes affected by the regulatory system, random lacZ fusions were generated in the genome of R. sphaeroides strain 2.4.1 using a promoter-trapping vector, pSG2. About 20,000 clones were screened and 23 showed a significantly different level of β-gal activities upon the addition of synthetic 7,8-cis-N-tetradecenoyl-homoserine lactone (RAI). Among these 23 clones, the clone showing the highest level of induction was selected for further study, where about a ten-fold increase of β-gal activity was exhibited in the presence of RAI and induction was shown to be required for cerR. In this clone, the 1acZ reporter was inserted in a putative gene that exhibited a low homology with catD. A genetic analysis showed that the expression of the catD homolog was initiated from a promoter of another gene present upstream of the catD. This upstream gene showed a strong homology with luxR and hence was named qsrR (quorum-sensing regulation regulator). A comparison of the total protein expression profiles for the wild-type cells and qsrR-null mutant cells using two-dimensional gel electrophoresis and a MALDI-TOF analysis allowed the identification of sets of genes modulated by the luxR homolog.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleGenes of Rhodobacter sphaeroides 2.4.1 regulated by innate quorum-sensing signal, 7,8-cis-N-(tetradecenoyl) homoserine lactone-
dc.title.alternativeGenes of Rhodobacter sphaeroides 2.4.1 regulated by innate quorum-sensing signal, 7,8-cis-N-(tetradecenoyl) homoserine lactone-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number2-
dc.citation.endPage227-
dc.citation.startPage219-
dc.citation.volume18-
dc.contributor.affiliatedAuthorByoung Chul Park-
dc.contributor.alternativeName황원-
dc.contributor.alternativeName이고은-
dc.contributor.alternativeName이정국-
dc.contributor.alternativeName박병철-
dc.contributor.alternativeName김근수-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 18, no. 2, pp. 219-227-
dc.subject.keywordquorum sensing-
dc.subject.keywordreporter vectors-
dc.subject.keywordrhodobacter sphaeroides-
dc.subject.localquorum sensing-
dc.subject.localQuorum sensing-
dc.subject.localreporter vectors-
dc.subject.localrhodobacter sphaeroides-
dc.description.journalClassY-
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Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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