Isolation and characterization of marine bacterial strain degrading fucoidan from Korean Undaria pinnatifida sporophylls

Cited 0 time in scopus
Metadata Downloads
Title
Isolation and characterization of marine bacterial strain degrading fucoidan from Korean Undaria pinnatifida sporophylls
Author(s)
W J Kim; S M Kim; Y H Lee; H G Kim; H K Kim; Seong Hoon Moon; H H Suh; K H Jang; Y I Park
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 18, no. 4, pp. 616-623
Publication Year
2008
Abstract
In spite of an increasing interest in fucoidans as biologically active compounds, no convenient commercial sources with fucoidanase activity are yet available. A marine bacterial strain that showed confluent growth on a minimal medium containing fucoidan, prepared from Korean Undaria pinnatifida sporophylls, as the sole carbon source was isolated and identified based on a 16S rDNA sequence analysis as a strain of Sphingomonas paucimobilis, and named Sphingomonas paucimobilis PF-1. The strain depolymerized facoidan into more than 7 distinct low-molecular-mass fucose-containing oligosaccharides, ranging from 305 to 3,749 Da. The enzyme activity was shown to be associated with the whole cell, suggesting the possibility of a surface display of the enzyme. However, a whole-cell enzyme preparation neither released the monomer L-fucose from the facoidan nor hydrolyzed the chromogenic substrate p-nitrophenyl-α-L-fucoside, indicating that the enzyme may be an endo-acting fucoidanase rather than an α-L-fucosidase. Therefore, this would appear to be the first report on facoidanolytic activity by a Sphingomonas species and also the first report on the enzymatic degradation of the Korean Undaria pinnatifida sporophyll fucoidan. Moreover, this enzyme activity may be very useful for structural analyses of fucose-containing polysaccharides and the production of bioactive fucooligosaccharides.
Keyword
fucoidanfucoidanasefucooligosaccharidesmarine bacterium
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.