DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jae Won Huh | - |
dc.contributor.author | H S Ha | - |
dc.contributor.author | Dae Soo Kim | - |
dc.contributor.author | H S Kim | - |
dc.date.accessioned | 2017-04-19T09:11:13Z | - |
dc.date.available | 2017-04-19T09:11:13Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0143-4004 | - |
dc.identifier.uri | 10.1016/j.placenta.2008.04.002 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8521 | - |
dc.description.abstract | Domestication events of long terminal repeat (LTR) sequences of the human endogenous retrovirus (HERV) family have been considered to be a new mechanism for the generation of alternative splicing in the human genome. We investigated an LTR10A belonging to the HERV-I family at the human endothelial nitric oxide synthase (NOS3) gene locus. The LTR10A element was located upstream of the original promoter sequences of NOS3. Expression analysis using RT-PCR and reporter gene assays in HCT116 and COS7 cells indicated placenta-specific expression of NOS3 driven by the LTR10A-derived promoter. The placenta-restricted expression was also determined to be associated with hypomethylation of the LTR10A element by methylation analysis using sodium bisulfite DNA sequencing. Furthermore, treatment of brain-derived cell lines with demethylation reagents did not restore expression of the LTR-derived NOS3 gene transcript. Taken together, the integration event of an LTR10A element in the upstream region of NOS3 led to the generation of a placenta-specific alternative transcript governed by cooperative mechanisms of epigenetic control (DNA methylation) and transcriptional regulation (interaction between cis- and trans-acting elements). | - |
dc.publisher | Elsevier | - |
dc.title | Placenta-restricted expression of LTR-derived NOS3 | - |
dc.title.alternative | Placenta-restricted expression of LTR-derived NOS3 | - |
dc.type | Article | - |
dc.citation.title | Placenta | - |
dc.citation.number | 7 | - |
dc.citation.endPage | 608 | - |
dc.citation.startPage | 602 | - |
dc.citation.volume | 29 | - |
dc.contributor.affiliatedAuthor | Jae Won Huh | - |
dc.contributor.affiliatedAuthor | Dae Soo Kim | - |
dc.contributor.alternativeName | 허재원 | - |
dc.contributor.alternativeName | 하홍석 | - |
dc.contributor.alternativeName | 김대수 | - |
dc.contributor.alternativeName | 김희수 | - |
dc.identifier.bibliographicCitation | Placenta, vol. 29, no. 7, pp. 602-608 | - |
dc.identifier.doi | 10.1016/j.placenta.2008.04.002 | - |
dc.subject.keyword | LTR10A | - |
dc.subject.keyword | Methylation | - |
dc.subject.keyword | NOS3 | - |
dc.subject.keyword | Placenta | - |
dc.subject.keyword | Reporter gene assay | - |
dc.subject.local | LTR10A | - |
dc.subject.local | methylation | - |
dc.subject.local | Methylation | - |
dc.subject.local | NOS3 | - |
dc.subject.local | Placenta | - |
dc.subject.local | placenta | - |
dc.subject.local | Reporter gene assay | - |
dc.description.journalClass | Y | - |
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