Placenta-restricted expression of LTR-derived NOS3

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dc.contributor.authorJae Won Huh-
dc.contributor.authorH S Ha-
dc.contributor.authorDae Soo Kim-
dc.contributor.authorH S Kim-
dc.date.accessioned2017-04-19T09:11:13Z-
dc.date.available2017-04-19T09:11:13Z-
dc.date.issued2008-
dc.identifier.issn0143-4004-
dc.identifier.uri10.1016/j.placenta.2008.04.002ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8521-
dc.description.abstractDomestication events of long terminal repeat (LTR) sequences of the human endogenous retrovirus (HERV) family have been considered to be a new mechanism for the generation of alternative splicing in the human genome. We investigated an LTR10A belonging to the HERV-I family at the human endothelial nitric oxide synthase (NOS3) gene locus. The LTR10A element was located upstream of the original promoter sequences of NOS3. Expression analysis using RT-PCR and reporter gene assays in HCT116 and COS7 cells indicated placenta-specific expression of NOS3 driven by the LTR10A-derived promoter. The placenta-restricted expression was also determined to be associated with hypomethylation of the LTR10A element by methylation analysis using sodium bisulfite DNA sequencing. Furthermore, treatment of brain-derived cell lines with demethylation reagents did not restore expression of the LTR-derived NOS3 gene transcript. Taken together, the integration event of an LTR10A element in the upstream region of NOS3 led to the generation of a placenta-specific alternative transcript governed by cooperative mechanisms of epigenetic control (DNA methylation) and transcriptional regulation (interaction between cis- and trans-acting elements).-
dc.publisherElsevier-
dc.titlePlacenta-restricted expression of LTR-derived NOS3-
dc.title.alternativePlacenta-restricted expression of LTR-derived NOS3-
dc.typeArticle-
dc.citation.titlePlacenta-
dc.citation.number7-
dc.citation.endPage608-
dc.citation.startPage602-
dc.citation.volume29-
dc.contributor.affiliatedAuthorJae Won Huh-
dc.contributor.affiliatedAuthorDae Soo Kim-
dc.contributor.alternativeName허재원-
dc.contributor.alternativeName하홍석-
dc.contributor.alternativeName김대수-
dc.contributor.alternativeName김희수-
dc.identifier.bibliographicCitationPlacenta, vol. 29, no. 7, pp. 602-608-
dc.identifier.doi10.1016/j.placenta.2008.04.002-
dc.subject.keywordLTR10A-
dc.subject.keywordMethylation-
dc.subject.keywordNOS3-
dc.subject.keywordPlacenta-
dc.subject.keywordReporter gene assay-
dc.subject.localLTR10A-
dc.subject.localmethylation-
dc.subject.localMethylation-
dc.subject.localNOS3-
dc.subject.localPlacenta-
dc.subject.localplacenta-
dc.subject.localReporter gene assay-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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