Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses

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dc.contributor.authorL Tang-
dc.contributor.authorMyoung Duck Kim-
dc.contributor.authorKyoung-Sil Yang-
dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorS H Kim-
dc.contributor.authorJ S Kim-
dc.contributor.authorD J Yun-
dc.contributor.authorSang Soo Kwak-
dc.contributor.authorHaeng Soon Lee-
dc.date.accessioned2017-04-19T09:11:13Z-
dc.date.available2017-04-19T09:11:13Z-
dc.date.issued2008-
dc.identifier.issn09628819-
dc.identifier.uri10.1007/s11248-007-9155-2ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8522-
dc.description.abstractIn plants, nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes. In this study, we developed transgenic potato plants (Solanum tuberosum L. cv. Atlantic) expressing Arabidopsis NDPK2 (AtNDPK2) gene in cytosols under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SN plants) or enhanced CaMV 35S promoter (EN plants) and evaluated their tolerance to various environmental stress, including methyl viologen (MV)-mediated oxidative stress, high temperature, and salt stress. When 250 μM MV was sprayed to whole plants, plants expressing NDPK2 showed significantly an enhanced tolerance compared to non-transgenic (NT) plants. SN plants and EN plants showed 51% and 32% less visible damage than NT plants, respectively. Transcript level of AtNDPK2 gene and NDPK2 activity in SN plants following MV treatment well reflected the plant phenotype. Ascorbate peroxidase (APX) activity was also increased in MV-treated SN plants. In addition, SN plants showed enhanced tolerance to high temperature at 42°C. The photosynthetic activity of SN plants after treatment of high temperature was decreased by about 10% compared to the plants grown at 25°C, whereas that of NT plants declined by 30%. When treated with 80 mM NaCl onto the plantlets, both SN plants and EN plants also showed a significant reduced damage in root growth. These results indicate that overexpression of NDPK2 under the stress-inducible SWPA2 promoter might efficiently regulate the oxidative stress derived from various environmental stresses.-
dc.publisherSpringer-
dc.titleEnhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses-
dc.title.alternativeEnhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses-
dc.typeArticle-
dc.citation.titleTransgenic Research-
dc.citation.number4-
dc.citation.endPage715-
dc.citation.startPage705-
dc.citation.volume17-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.alternativeNameTang-
dc.contributor.alternativeName김명덕-
dc.contributor.alternativeName양경실-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName김선형-
dc.contributor.alternativeName김진석-
dc.contributor.alternativeName윤대진-
dc.contributor.alternativeName곽상수-
dc.contributor.alternativeName이행순-
dc.identifier.bibliographicCitationTransgenic Research, vol. 17, no. 4, pp. 705-715-
dc.identifier.doi10.1007/s11248-007-9155-2-
dc.subject.keywordAscorbate peroxidase-
dc.subject.keywordHigh temperature-
dc.subject.keywordMolecular breeding-
dc.subject.keywordOxidative stress-
dc.subject.keywordPhotosynthetic activity-
dc.subject.keywordSalt stress-
dc.subject.localAscorbate peroxidase-
dc.subject.localHigh temperature-
dc.subject.localMolecular breeding-
dc.subject.localOxidative stress-
dc.subject.localPhotosynthetic activity-
dc.subject.localSalt stress-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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