Overexpression of a new rice vacuolar antiporter regulating protein OsARP improves salt tolerance in tobacco

Cited 26 time in scopus
Metadata Downloads
Title
Overexpression of a new rice vacuolar antiporter regulating protein OsARP improves salt tolerance in tobacco
Author(s)
M I Uddin; Y Qi; S Yamada; I Shibuya; X P Deng; Sang Soo Kwak; H Kaminaka; K Tanaka
Bibliographic Citation
Plant and Cell Physiology, vol. 49, no. 6, pp. 880-890
Publication Year
2008
Abstract
We examined the function of the rice (Oryza sativa L.) antiporter- regulating protein OsARP by overexpressing it in tobacco (Nicotiana tabacum L.). In public databases, this protein was annotated as a putative Os02g0465900 protein of rice. The OsARP gene was introduced into tobacco under the control of the cauliflower mosaic virus 35S promoter. The transformants were selected for their ability to grow on medium containing kanamycin. Incorporation of the transgene in the genome of tobacco was confirmed by PCR, and its expression was confirmed by Western blot analysis. Transgenic plants had better growth and vigor than non-transgenic plants under salt stress in vitro. Overexpression of OsARP in transgenic tobacco plants resulted in salt tolerance, and the plants had a higher rate of photosynthesis and effective PSII photon yield when compared with the wild type. The OsARP protein was localized in the tonoplast of rice plants. Transgenic plants accumulated more Na+ in their leaf tissue than did wild-type plants. It is conceivable that the toxic effect of Na+ in the cytosol might be reduced by sequestration into vacuoles. The rate of water loss was higher in the wild type than in transgenic plants under salt stress. Increased vacuolar solute accumulation and water retention could confer salt tolerance in transgenic plants. Tonoplast vesicles isolated from OsARP transgenic plants showed Na+/H+ exchange rates 3-fold higher than those of wild-type plants. These results suggest that OsARP on the tonoplasts plays an important role in compartmentation of Na+ into vacuoles. We suggest that OsARP is a new type of protein participating in Na+ uptake in vacuoles.
Keyword
Na+/H+ exchangeRice (Oryza sativa L.)Salt toleranceTobacco (Nicotiana tabacum L.)Vacuolar antiporter regulator
ISSN
0032-0781
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/pcp/pcn062
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.