DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seung Kyoon Kim | - |
dc.contributor.author | Hay Ran Jang | - |
dc.contributor.author | Jeong Hwan Kim | - |
dc.contributor.author | Mirang Kim | - |
dc.contributor.author | S M Noh | - |
dc.contributor.author | K S Song | - |
dc.contributor.author | G H Kang | - |
dc.contributor.author | H J Kim | - |
dc.contributor.author | Seon-Young Kim | - |
dc.contributor.author | Hyang Sook Yoo | - |
dc.contributor.author | Yong Sung Kim | - |
dc.date.accessioned | 2017-04-19T09:11:23Z | - |
dc.date.available | 2017-04-19T09:11:23Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0143-3334 | - |
dc.identifier.uri | 10.1093/carcin/bgn110 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8537 | - |
dc.description.abstract | Transcriptional factor 4 (TCF4), encoding a basic helix-loop-helix transcriptional factor, has recently been demonstrated as a causative gene for Pitt-Hopkins syndrome, a neurodevelopmental disease. Examination of gastric cancers using the restriction landmark genomic scanning technique revealed methylation at a NotI enzyme site in TCF4 intron 8 and further identified CpG dinucleotide hypermethylation in TCF4 exon 1, strongly associated with gene silencing in gastric cancer cell lines. Treatment with 5-aza-2′-deoxycytidine and/or trichostatin A restored TCF4 expression in TCF4-silenced gastric cancer cell lines. Real-time reverse transcription-polymerase chain reaction analysis of 77 paired primary gastric tumor samples revealed that 38% of analyzed tumors had a >2-fold decrease in TCF4 expression compared with adjacent normal-appearing tissue, and the decrease significantly correlated with increased CpG methylation in TCF4 exon 1. Clinicopathologic data showed that decreased TCF4 expression occurred significantly more frequently in intestinal-type (22/37, 59%) than in diffuse-type (7/37, 19%) gastric cancers (P = 0.0004) and likewise more frequently in early (12/18, 67%) than in advanced (17/59, 29%) gastric cancers (P = 0.004). CpG methylation markedly increased with patient age among normal-appearing tissues, suggesting that CpG methylation in gastric mucosa may be one of the earliest events in carcinogenesis of intestinal-type gastric cancers. Furthermore, ectopic expression of TCF4 decreased cell growth in a gastric cancer cell line, and the knock down of TCF4 using small interfering RNA increased cell migration. Based on these results, we propose that the observed frequent epigenetic-mediated TCF4 silencing plays a role in tumor formation and progression. | - |
dc.publisher | Oxford Univ Press | - |
dc.title | CpG methylation in exon 1 of transcription factor 4 increases with age in normal gastric mucosa and is associated with gene silencing in intestinal-type gastric cancers | - |
dc.title.alternative | CpG methylation in exon 1 of transcription factor 4 increases with age in normal gastric mucosa and is associated with gene silencing in intestinal-type gastric cancers | - |
dc.type | Article | - |
dc.citation.title | Carcinogenesis | - |
dc.citation.number | 8 | - |
dc.citation.endPage | 1631 | - |
dc.citation.startPage | 1623 | - |
dc.citation.volume | 29 | - |
dc.contributor.affiliatedAuthor | Seung Kyoon Kim | - |
dc.contributor.affiliatedAuthor | Hay Ran Jang | - |
dc.contributor.affiliatedAuthor | Jeong Hwan Kim | - |
dc.contributor.affiliatedAuthor | Mirang Kim | - |
dc.contributor.affiliatedAuthor | Seon-Young Kim | - |
dc.contributor.affiliatedAuthor | Hyang Sook Yoo | - |
dc.contributor.affiliatedAuthor | Yong Sung Kim | - |
dc.contributor.alternativeName | 김승균 | - |
dc.contributor.alternativeName | 장해란 | - |
dc.contributor.alternativeName | 김정환 | - |
dc.contributor.alternativeName | 김미랑 | - |
dc.contributor.alternativeName | 노승무 | - |
dc.contributor.alternativeName | 송규상 | - |
dc.contributor.alternativeName | 강경훈 | - |
dc.contributor.alternativeName | 김희진 | - |
dc.contributor.alternativeName | 김선영 | - |
dc.contributor.alternativeName | 유향숙 | - |
dc.contributor.alternativeName | 김용성 | - |
dc.identifier.bibliographicCitation | Carcinogenesis, vol. 29, no. 8, pp. 1623-1631 | - |
dc.identifier.doi | 10.1093/carcin/bgn110 | - |
dc.description.journalClass | Y | - |
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