Development of microbial genome-probing microarrays using digital multiple displacement amplification of uncultivated microbial single cells = 미배양 단일 세포의 디지털 다중증폭법을 통한 미배양 미생물 모니터링용 GPM 개발

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dc.contributor.authorHo Won Chang-
dc.contributor.authorY Sung-
dc.contributor.authorKyoung-Ho Kim-
dc.contributor.authorYoung Do Nam-
dc.contributor.authorSeong Woon Roh-
dc.contributor.authorMin-Su Kim-
dc.contributor.authorC O Jeon-
dc.contributor.authorJin-Woo Bae-
dc.date.accessioned2017-04-19T09:11:23Z-
dc.date.available2017-04-19T09:11:23Z-
dc.date.issued2008-
dc.identifier.issn0013-936X-
dc.identifier.uri10.1021/es8006029ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8540-
dc.description.abstractA crucial problem in the use of previously developed genome-probing microarrays (GPM) has been the inability to use uncultivated bacterial genomes to take advantage of the high sensitivity and specificity of GPM in microbial detection and monitoring. We show here a method, digital multiple displacement amplification (MDA), to amplify and analyze various genomes obtained from single uncultivated bacterial cells. We used 15 genomes from key microbes involved in dichloromethane (DCM)-dechlorinating enrichment as microarray probes to uncover the bacterial population dynamics of samples without PCR amplification. Genomic DNA amplified from single cells originating from uncultured bacteria with 80.3-99.4% similarity to 16S rRNA genes of cultivated bacteria. The digital MDA-GPM method successfully monitored the dynamics of DCM-dechlorinating communities from different phases of enrichment status. Without a priori knowledge of microbial diversity, the digital MDA-GPM method could be designed to monitor most microbial populations in a given environmental sample.-
dc.publisherAmer Chem Soc-
dc.titleDevelopment of microbial genome-probing microarrays using digital multiple displacement amplification of uncultivated microbial single cells = 미배양 단일 세포의 디지털 다중증폭법을 통한 미배양 미생물 모니터링용 GPM 개발-
dc.title.alternativeDevelopment of microbial genome-probing microarrays using digital multiple displacement amplification of uncultivated microbial single cells-
dc.typeArticle-
dc.citation.titleEnvironmental Science & Technology-
dc.citation.number16-
dc.citation.endPage6064-
dc.citation.startPage6058-
dc.citation.volume42-
dc.contributor.affiliatedAuthorHo Won Chang-
dc.contributor.affiliatedAuthorKyoung-Ho Kim-
dc.contributor.affiliatedAuthorYoung Do Nam-
dc.contributor.affiliatedAuthorSeong Woon Roh-
dc.contributor.affiliatedAuthorMin-Su Kim-
dc.contributor.affiliatedAuthorJin-Woo Bae-
dc.contributor.alternativeName장호원-
dc.contributor.alternativeName성열붕-
dc.contributor.alternativeName김경호-
dc.contributor.alternativeName남영도-
dc.contributor.alternativeName노성운-
dc.contributor.alternativeName김민수-
dc.contributor.alternativeName전체옥-
dc.contributor.alternativeName배진우-
dc.identifier.bibliographicCitationEnvironmental Science & Technology, vol. 42, no. 16, pp. 6058-6064-
dc.identifier.doi10.1021/es8006029-
dc.description.journalClassY-
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