Nanoscopic observation of a gold nanoparticle-conjugated protein using near-field scanning optical microscopy

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dc.contributor.authorHyun Kyu Park-
dc.contributor.authorYong Taik Lim-
dc.contributor.authorJin Kyeong Kim-
dc.contributor.authorH G Park-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T09:11:27Z-
dc.date.available2017-04-19T09:11:27Z-
dc.date.issued2008-
dc.identifier.issn0304-3991-
dc.identifier.uri10.1016/j.ultramic.2008.04.024ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8555-
dc.description.abstractThis study describes a single gold nanoparticle (AuNP)-based observation of biomolecular interaction using a near-field scanning microscope (NSOM) in transmission mode. To observe streptavidin molecules, a glass surface was first patterned with a micro-scale line of (3-aminopropyl)trimethoxysilane (APTMS) by micro-contact printing (μCP) with a subsequent reaction of N-hydroxysuccinimide (NHS)-biotin. The AuNP-conjugated streptavidin was then applied to the biotin-modified glass surface and NSOM was employed to detect the resulting specific interaction between streptavidin and biotin on the glass surface. Using the optical and topological images generated from the NSOM analysis, the interaction could be observed at the nanoscopic scale. This study demonstrates that the NSOM is a powerful tool for the detection of protein interactions at the nanoscopic level when the protein is conjugated with AuNPs.-
dc.publisherElsevier-
dc.titleNanoscopic observation of a gold nanoparticle-conjugated protein using near-field scanning optical microscopy-
dc.title.alternativeNanoscopic observation of a gold nanoparticle-conjugated protein using near-field scanning optical microscopy-
dc.typeArticle-
dc.citation.titleUltramicroscopy-
dc.citation.number10-
dc.citation.endPage1119-
dc.citation.startPage1115-
dc.citation.volume108-
dc.contributor.affiliatedAuthorHyun Kyu Park-
dc.contributor.affiliatedAuthorYong Taik Lim-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName박현규-
dc.contributor.alternativeName임용택-
dc.contributor.alternativeName김진경-
dc.contributor.alternativeName박현규-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationUltramicroscopy, vol. 108, no. 10, pp. 1115-1119-
dc.identifier.doi10.1016/j.ultramic.2008.04.024-
dc.subject.keywordGold nanoparticle (AuNP)-
dc.subject.keywordNear-field scanning optical microscopy (NSOM)-
dc.subject.keywordStreptavidin detection-
dc.subject.localgold nanoparticle-
dc.subject.localGold nanoparticles-
dc.subject.localGold nanoparticle (AuNP)-
dc.subject.localGold nanoparticle-
dc.subject.localNear-field scanning optical microscopy (NSOM)-
dc.subject.localStreptavidin detection-
dc.description.journalClassY-
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