Interactome analysis of yeast glutathione peroxidase 3 = 효모 GPx3의 사호작용단백질체학 분석

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Title
Interactome analysis of yeast glutathione peroxidase 3 = 효모 GPx3의 사호작용단백질체학 분석
Author(s)
Phil Young Lee; Kwang-Hee Bae; Chang Won Kho; Sunghyun Kang; D H Lee; S Cho; S Kang; Sang Chul LeeByoung Chul ParkSung Goo Park
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 18, no. 8, pp. 1364-1367
Publication Year
2008
Abstract
Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.
Keyword
glutathione peroxidase 3IP-2DEoxidative stressROSSSY protein 3yeastglutathione peroxidase
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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