Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression

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dc.contributor.authorJ I Chae-
dc.contributor.authorSeong Kyu Joo-
dc.contributor.authorMyung Kyu Lee-
dc.contributor.authorJ H Park-
dc.contributor.authorJ H Shim-
dc.contributor.authorKyung Kwang Lee-
dc.contributor.authorD S Lee-
dc.date.accessioned2017-04-19T09:11:37Z-
dc.date.available2017-04-19T09:11:37Z-
dc.date.issued2008-
dc.identifier.issn00268933-
dc.identifier.uri10.1134/S0026893308040122ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8571-
dc.description.abstractThymus-and activation-regulated chemokine (TARC) is one that selectively controls the migration of type 2-helper T lymphocytes into inflammatory lesions. TARC is a CC chemokine and plays an essential role in recruiting CC chemokine receptor 4-positive Th2 cells to allergic lesions. We cloned TARC cDNA from rat thymus using RT-PCR. The rat TARC clone contained a full-length open reading frame encoding 93 amino acids that showed 83 and 66% homology with mouse and human homologs, respectively. The expression of TARC mRNA was mainly in the lymphoid organs, for example, the thymus, spleen, and lymph node. The recombinant TARC was expressed in Escherichia coli and purified in an active form. In addition, the purified rat TARC with S-tagged specifically binds to human CCR4 in CD4/CCR4-transfected HOS cells by cell-binding assay using flow cytometry. The TARC cDNA clones obtained in this study will be valuable for future studies on allergic diseases in rats.-
dc.publisherSpringer-
dc.titleCloning of rat TARC cDNA and analysis of tissue-specific mRNA expression-
dc.title.alternativeCloning of rat TARC cDNA and analysis of tissue-specific mRNA expression-
dc.typeArticle-
dc.citation.titleMolecular Biology-
dc.citation.number4-
dc.citation.endPage571-
dc.citation.startPage567-
dc.citation.volume42-
dc.contributor.affiliatedAuthorMyung Kyu Lee-
dc.contributor.alternativeName채정일-
dc.contributor.alternativeName주성규-
dc.contributor.alternativeName이명규-
dc.contributor.alternativeName박정현-
dc.contributor.alternativeName심정현-
dc.contributor.alternativeName이경광-
dc.contributor.alternativeName이동석-
dc.identifier.bibliographicCitationMolecular Biology, vol. 42, no. 4, pp. 567-571-
dc.identifier.doi10.1134/S0026893308040122-
dc.subject.keywordCCR4-
dc.subject.keywordChemokine-
dc.subject.keywordImmunological regulation-
dc.subject.keywordMolecular immunoligy-
dc.subject.keywordTARC-
dc.subject.localCCR4-
dc.subject.localChemokine-
dc.subject.localImmunological regulation-
dc.subject.localMolecular immunoligy-
dc.subject.localTARC-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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