Amplification of uncultured single-stranded DNA viruses from rice paddy soil

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dc.contributor.authorKyung Ho Kim-
dc.contributor.authorHo Won Chang-
dc.contributor.authorYoung Do Nam-
dc.contributor.authorSeong Woon Roh-
dc.contributor.authorMin-Su Kim-
dc.contributor.authorY Sung-
dc.contributor.authorC O Jeon-
dc.contributor.authorHee-Mock Oh-
dc.contributor.authorJin-Woo Bae-
dc.date.accessioned2017-04-19T09:11:43Z-
dc.date.available2017-04-19T09:11:43Z-
dc.date.issued2008-
dc.identifier.issn0099-2240-
dc.identifier.uri10.1128/AEM.01275-08ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8600-
dc.description.abstractViruses are known to be the most numerous biological entities in soil; however, little is known about their diversity in this environment. In order to explore the genetic diversity of soil viruses, we isolated viruses by centrifugation and sequential filtration before performing a metagenomic investigation. We adopted multiple-displacement amplification (MDA), an isothermal whole-genome amplification method with φ29 polymerase and random hexamers, to amplify viral DNA and construct clone libraries for metagenome sequencing. By the MDA method, the diversity of both single-stranded DNA (ssDNA) viruses and double-stranded DNA viruses could be investigated at the same time. On the contrary, by eliminating the denaturing step in the MDA reaction, only ssDNA viral diversity could be explored selectively. Irrespective of the denaturing step, more than 60% of the soil metagenome sequences did not show significant hits (E-value criterion, 0.001) with previously reported viral sequences. Those hits that were considered to be significant were also distantly related to known ssDNA viruses (average amino acid similarity, approximately 34%). Phylogenetic analysis showed that replication-related proteins (which were the most frequently detected proteins) related to those of ssDNA viruses obtained from the metagenomic sequences were diverse and novel. Putative circular genome components of ssDNA viruses that are unrelated to known viruses were assembled from the metagenomic sequences. In conclusion, ssDNA viral diversity in soil is more complex than previously thought. Soil is therefore a rich pool of previously unknown ssDNA viruses.-
dc.publisherAmer Soc Microb-
dc.titleAmplification of uncultured single-stranded DNA viruses from rice paddy soil-
dc.title.alternativeAmplification of uncultured single-stranded DNA viruses from rice paddy soil-
dc.typeArticle-
dc.citation.titleApplied and Environmental Microbiology-
dc.citation.number19-
dc.citation.endPage5985-
dc.citation.startPage5975-
dc.citation.volume74-
dc.contributor.affiliatedAuthorKyung Ho Kim-
dc.contributor.affiliatedAuthorHo Won Chang-
dc.contributor.affiliatedAuthorYoung Do Nam-
dc.contributor.affiliatedAuthorSeong Woon Roh-
dc.contributor.affiliatedAuthorMin-Su Kim-
dc.contributor.affiliatedAuthorHee-Mock Oh-
dc.contributor.affiliatedAuthorJin-Woo Bae-
dc.contributor.alternativeName김경호-
dc.contributor.alternativeName장호원-
dc.contributor.alternativeName남영도-
dc.contributor.alternativeName노성운-
dc.contributor.alternativeName김민수-
dc.contributor.alternativeName성열붕-
dc.contributor.alternativeName전체옥-
dc.contributor.alternativeName오희목-
dc.contributor.alternativeName배진우-
dc.identifier.bibliographicCitationApplied and Environmental Microbiology, vol. 74, no. 19, pp. 5975-5985-
dc.identifier.doi10.1128/AEM.01275-08-
dc.description.journalClassY-
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Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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