The efficiency of RNA interference in Bursaphelenchus xylophilus

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Title
The efficiency of RNA interference in Bursaphelenchus xylophilus
Author(s)
J E Park; K Y Lee; S J Lee; W S Oh; P Y Jeong; Taeha Woo; Chang-Bae Kim; Y K Paik; H S Koo
Bibliographic Citation
Molecules and Cells, vol. 26, no. 1, pp. 81-86
Publication Year
2008
Abstract
RNA Interference (RNAI) was performed on several essential genes in the pinewood nematode Bursaphelenchus xylophilus, which causes pine wilt disease. Double-stranded RNA (deRNA) was delivered to larvae or adult worms by soaking, electroporation, or microinjection. Soaking and electroporation of L2-L3 stage worms in solutions containing dsRNA for essential genes Induced over 250% lethality after 5 days, and gene-specific phenotypes were observed. This lethality agreed with significant reductions of the targeted transcripts, as assayed by reverse-transcription coupled with real time PCR. Microinjection was the most efficient routs as measured by the hatching rate of F1 embryos, which was reduced by 46%. When adult worms were soaked In dsRNA, lethality was induced in the F1 larvae, revealing the persistence of knockdown phenotypes. The penstrance of the RNA/phenotypes for essential genes was relatively low but consistent indicating that RNA/ should be useful for studying the in vivo functions of B. xylophilus gene products.
Keyword
cytochrome Cheat shock protein 70myosin heavy chainpinewood nematodeRNAitropomyosin
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
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1. Journal Articles > Journal Articles
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