Sialic acids in Trichoplusia ni and Danaus plexippus egg glycoproteins

Cited 0 time in scopus
Metadata Downloads
Title
Sialic acids in Trichoplusia ni and Danaus plexippus egg glycoproteins
Author(s)
Yong Il Park; Byung Dae Yoon; Y C Lee
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 4, no. 3, pp. 165-169
Publication Year
1999
Abstract
Baculovirus expression vector insect cell system (BEVIS) is useful for high level production of human therapeutic proteins. However, it has been reported that recombinant glycoproteins produced from this system are, in many cases, biologically inactive or less active than authentic counterparts, due to incomplete glycosylation potential of insect cells used so far, producing recombinant proteins with only high- or paucimannosidic oligosaccharides without sialylation. The presence of sialic acids in insects is still controversial. Egg proteins of Trichoplusia ni and Danaus plexippus were isolated, and the presence of sialic acids was examined using reverse-phase fluorescent HPLC after derivatization of samples with 1,2-diamino-4,5-methylenedioxybenzene (DMB). The proteins of both eggs were shown to contain 5-N-acetylneuraminic acid. The results suggest that both insects may be able to produce proteins with sialylated complex-type oligosaccharide chains.
Keyword
carbohydratesglycoproteinsHPLCinsectssialic acids
ISSN
1226-8372
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF02931922
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.