Cooperative exonization of MaLR and AluJo elements contributed an alternative promoter and novel splice variants of RNF19 = MaLR 과 ALuJo의 엑소나이제이션 기작이 RNF19의 얼터너티브 프로모터와 스플라이싱 variants의 형성에 기여

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Title
Cooperative exonization of MaLR and AluJo elements contributed an alternative promoter and novel splice variants of RNF19 = MaLR 과 ALuJo의 엑소나이제이션 기작이 RNF19의 얼터너티브 프로모터와 스플라이싱 variants의 형성에 기여
Author(s)
Jae Won HuhDae Soo Kim; H S Ha; J R Lee; Y J Kim; K Ahn; Sang Rae Lee; Kyu Tae Chang; H S Kim
Bibliographic Citation
Gene, vol. 424, no. 1, pp. 63-70
Publication Year
2008
Abstract
The RNF19 protein, which contains RING-finger and IBR motifs, acts as an E3 ubiquitin ligase localized to Lewy bodies. RNF19 is located on human chromosome 8q22.2, has a 4.4-kb transcript, and is ubiquitously expressed in various tissues. Here, we identified an alternative RNF19 promoter region and alternative RNF19 transcripts derived from MaLR (mammalian apparent LTR-retrotransposon) and AluJo elements. Comparative analyses indicated human-specific expression of the MaLR- and AluJo-related transcripts. From the expression analysis of 72 tissue samples including human normal, tumor, and primate tissues, three different isoforms (V1, V2, and V3) of MaLR-derived transcripts were identified. Quantitative RT-PCR analysis showed a dominant expression pattern of the V2 MaLR-derived transcript. A reporter gene assay for MaLR element promoter activity indicated that pGL2-RNF19/MaLR in the forward orientation is capable of driving luciferase gene expression in Cos7 and HCT116 cells. These findings suggest that RNF19 has acquired a new promoter and alternative exons via continuous retrotransposition events of MaLR and AluJo elements during mammalian and primate evolution, respectively.
Keyword
AluJoMaLRMammalian evolutionPromoter activityRNF19
ISSN
0378-1119
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.gene.2008.07.030
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
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