Silencing of a BYPASS1 homolog results in root-independent pleiotrophic developmental defects in Nicotiana benthamiana

Cited 12 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorY W Kang-
dc.contributor.authorRyong Nam Kim-
dc.contributor.authorHye Sun Cho-
dc.contributor.authorW T Kim-
dc.contributor.authorD Choi-
dc.contributor.authorH S Pai-
dc.date.accessioned2017-04-19T09:11:55Z-
dc.date.available2017-04-19T09:11:55Z-
dc.date.issued2008-
dc.identifier.issn0167-4412-
dc.identifier.uri10.1007/s11103-008-9384-7ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8640-
dc.description.abstractThe Arabidopsis bypass1 mutant (bps1) exhibits defective shoot and root growth that is associated with constitutive production of a root-derived carotenoid-related signal (Van Norman et al., Curr Biol 14:1739-1746, 2004). Since the identity of the signal and the function of BPS1 are still unknown, we investigated effects of BPS1 depletion in Nicotiana benthamiana to elucidate BPS1 function in plant growth and development. The predicted protein of NbBPS1, a BPS1 homolog of N. benthamiana, contains a central transmembrane domain, and a NbBPS1:GFP fusion protein was mainly associated with the endoplasmic reticulum. Virus-induced gene silencing (VIGS) of NbBPS1 resulted in pleiotrophic phenotypes, including growth retardation and abnormal leaf development. At the cellular level, the plants exhibited hyperproliferation of the cambial cells and defective xylem differentiation during stem vascular development. Hyperactivity of the cambium was associated with an elevated auxin and cytokinin response. In contrast, the leaves had reduced numbers of cells with increased cell size and elevated endoreduplication. Cell death in NbBPS1 VIGS leaves started with vacuole collapse, followed by degeneration of the organelles. Interestingly, these phenotypes were mainly caused by silencing of NbBPS1 in the aerial parts of the plants, different from the case of the Arabidopsis bps1 mutant. These results suggest that NbBPS1 plays a role in the control of cell division and differentiation in the cambium of N. benthamiana, and BPS homologs may have a diverse function in different tissues and in different species.-
dc.publisherSpringer-
dc.titleSilencing of a BYPASS1 homolog results in root-independent pleiotrophic developmental defects in Nicotiana benthamiana-
dc.title.alternativeSilencing of a BYPASS1 homolog results in root-independent pleiotrophic developmental defects in Nicotiana benthamiana-
dc.typeArticle-
dc.citation.titlePlant Molecular Biology-
dc.citation.number4-
dc.citation.endPage437-
dc.citation.startPage423-
dc.citation.volume68-
dc.contributor.affiliatedAuthorRyong Nam Kim-
dc.contributor.affiliatedAuthorHye Sun Cho-
dc.contributor.alternativeName강용원-
dc.contributor.alternativeName김룡남-
dc.contributor.alternativeName조혜선-
dc.contributor.alternativeName김우택-
dc.contributor.alternativeName최도일-
dc.contributor.alternativeName배현숙-
dc.identifier.bibliographicCitationPlant Molecular Biology, vol. 68, no. 4, pp. 423-437-
dc.identifier.doi10.1007/s11103-008-9384-7-
dc.subject.keywordcell death-
dc.subject.keywordendoreduplication-
dc.subject.keywordER targeting-
dc.subject.keywordhyperproliferation-
dc.subject.keywordpromoter-GUS fusion-
dc.subject.keywordvirus-induced gene silencing-
dc.subject.localcell death-
dc.subject.localCell death-
dc.subject.localendoreduplication-
dc.subject.localER targeting-
dc.subject.localhyperproliferation-
dc.subject.localpromoter-GUS fusion-
dc.subject.localPromoter-GUS fusion-
dc.subject.localVirus-induced gene silencing (VIGS)-
dc.subject.localvirus-induced gene silencing-
dc.subject.localVirus-induced gene silencing-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.