Enhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster = 생합성 유전자의 프로모터 개량에 의한 이종숙주에서 EPA의 생산량 증대

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dc.contributor.authorSoo-Jin Lee-
dc.contributor.authorChul Ho Kim-
dc.contributor.authorPil-Soo Seo-
dc.contributor.authorOh Suk Kwon-
dc.contributor.authorB K Hur-
dc.contributor.authorJeong Woo Seo-
dc.date.accessioned2017-04-19T09:11:55Z-
dc.date.available2017-04-19T09:11:55Z-
dc.date.issued2008-
dc.identifier.issn0141-5492-
dc.identifier.uri10.1007/s10529-008-9809-zko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8643-
dc.description.abstractTo enhance the heterologous production of eicosapentaenoic acid (EPA) in Escherichia coli, the EPA biosynthesis gene cluster from Shewanella oneidensis MR-1 was cloned under the lacZ promoter on a high-copy number plasmid, pBluescript SK(+). The production of EPA was remarkably enhanced yielding levels of up to 7.5% of the total fatty acid content in the recombinant E. coli strain by induction with IPTG, whereas the stimulation of EPA production was abolished by adding glucose into the culture medium, probably due to glucose repression acting on the promoter activity.-
dc.publisherSpringer-
dc.titleEnhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster = 생합성 유전자의 프로모터 개량에 의한 이종숙주에서 EPA의 생산량 증대-
dc.title.alternativeEnhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number12-
dc.citation.endPage2142-
dc.citation.startPage2139-
dc.citation.volume30-
dc.contributor.affiliatedAuthorSoo-Jin Lee-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.affiliatedAuthorPil-Soo Seo-
dc.contributor.affiliatedAuthorOh Suk Kwon-
dc.contributor.affiliatedAuthorJeong Woo Seo-
dc.contributor.alternativeName이수진-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName서필수-
dc.contributor.alternativeName권오석-
dc.contributor.alternativeName허병기-
dc.contributor.alternativeName서정우-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 30, no. 12, pp. 2139-2142-
dc.identifier.doi10.1007/s10529-008-9809-z-
dc.subject.keywordBiosynthesis gene cluster-
dc.subject.keywordEicosapentaenoic acid-
dc.subject.keywordEscherichia coli-
dc.subject.keywordHeterologous production-
dc.subject.keywordLacZ promoter-
dc.subject.keywordShewanella oneidensis-
dc.subject.localBiosynthesis gene cluster-
dc.subject.localeicosapentaenoic acid (EPA)-
dc.subject.localEicosapentaenoic acid-
dc.subject.localEscherichia coli.-
dc.subject.localescherichia coli-
dc.subject.localEscherichia Coli-
dc.subject.localEscherichia coli-
dc.subject.localE.coli-
dc.subject.localescherichia coil-
dc.subject.localE. coli-
dc.subject.localE. Coli-
dc.subject.localHeterologous production-
dc.subject.localLacZ promoter-
dc.subject.localShewanella oneidensis-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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