A comparison of gene expression profiles between primary human AML cells and AML cell line

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dc.contributor.authorJ Lee-
dc.contributor.authorJ Hwang-
dc.contributor.authorH S Kim-
dc.contributor.authorS Kim-
dc.contributor.authorY H Kim-
dc.contributor.authorS Y Park-
dc.contributor.authorK S Kim-
dc.contributor.authorZ Y Ryoo-
dc.contributor.authorKyu Tae Chang-
dc.contributor.authorS Lee-
dc.date.accessioned2017-04-19T09:11:57Z-
dc.date.available2017-04-19T09:11:57Z-
dc.date.issued2008-
dc.identifier.issn1341-7568-
dc.identifier.uri10.1266/ggs.83.339ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8646-
dc.description.abstractIn acute myeloid leukemia (AML), hematologic malignancies are characterized by recurring chromosomal abnormalities. Chromosome translocation t(9;11)(p22;q23) is one of the most common genetic aberrations and results in the formation of the MLL-AF9 fusion gene that functions as a facilitator of cell growth directly. In order to study this type of AML, the cell lines with cytogenetically diagnosed t(9;11)(p22;q23), such as Mono Mac 6 (MM6), have been widely used. To examine whether there is any difference in gene expression between the primary human t(9;11) AML cells and MM6 cell line, genome-wide transcriptome analysis was performed on MM6 cell line using SAGE and the results were compared to the profile of primary human t(9;11) AML cells. 884 transcripts which were alternatively expressed between MM6 cells and primary human t(9;11) cells were identified through statistical analysis (P < 0.05) and 4-fold expression change. of these transcripts, 830 (94%) matched to known genes or EST were classified by functional categories (http://david.abcc. ncifcrf.gov/). The majority of alternatively expressed genes in MM6 were involved in biosynthetic and metabolic processes, but HRAS, a protein that is known to be associated with leukemogenesis, was expressed only in MM6 cells and several other genes involved in Erk1/Erk2 MAPK pathway were also over-expressed in MM6. Therefore, since MM6 cell line has a similar expression profile to primary human t(9;11) AML in general and expresses uniquely a strong Erk1/Erk2 MAPK pathway including HRAS, it can be used as a model for HRAS-positive t(9;11) AML.-
dc.publisherGenetics Soc Japan-
dc.titleA comparison of gene expression profiles between primary human AML cells and AML cell line-
dc.title.alternativeA comparison of gene expression profiles between primary human AML cells and AML cell line-
dc.typeArticle-
dc.citation.titleGenes & Genetic Systems-
dc.citation.number0-
dc.citation.endPage345-
dc.citation.startPage339-
dc.citation.volume83-
dc.contributor.affiliatedAuthorKyu Tae Chang-
dc.contributor.alternativeName이진석-
dc.contributor.alternativeName황준모-
dc.contributor.alternativeName김형수-
dc.contributor.alternativeName김성곤-
dc.contributor.alternativeName김영훈-
dc.contributor.alternativeName박소영-
dc.contributor.alternativeName김길수-
dc.contributor.alternativeName유재영-
dc.contributor.alternativeName장규태-
dc.contributor.alternativeName이상규-
dc.identifier.bibliographicCitationGenes & Genetic Systems, vol. 83, pp. 339-345-
dc.identifier.doi10.1266/ggs.83.339-
dc.subject.keywordAML-
dc.subject.keywordGene expression-
dc.subject.keywordSAGE-
dc.subject.keywordTranslocation-
dc.subject.localAML-
dc.subject.localGene Expression-
dc.subject.localGene expression-
dc.subject.localgene expression-
dc.subject.localSAGE-
dc.subject.localTranslocation-
dc.subject.localtranslocation-
dc.description.journalClassY-
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