DC Field | Value | Language |
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dc.contributor.author | J Lee | - |
dc.contributor.author | J Hwang | - |
dc.contributor.author | H S Kim | - |
dc.contributor.author | S Kim | - |
dc.contributor.author | Y H Kim | - |
dc.contributor.author | S Y Park | - |
dc.contributor.author | K S Kim | - |
dc.contributor.author | Z Y Ryoo | - |
dc.contributor.author | Kyu Tae Chang | - |
dc.contributor.author | S Lee | - |
dc.date.accessioned | 2017-04-19T09:11:57Z | - |
dc.date.available | 2017-04-19T09:11:57Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1341-7568 | - |
dc.identifier.uri | 10.1266/ggs.83.339 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8646 | - |
dc.description.abstract | In acute myeloid leukemia (AML), hematologic malignancies are characterized by recurring chromosomal abnormalities. Chromosome translocation t(9;11)(p22;q23) is one of the most common genetic aberrations and results in the formation of the MLL-AF9 fusion gene that functions as a facilitator of cell growth directly. In order to study this type of AML, the cell lines with cytogenetically diagnosed t(9;11)(p22;q23), such as Mono Mac 6 (MM6), have been widely used. To examine whether there is any difference in gene expression between the primary human t(9;11) AML cells and MM6 cell line, genome-wide transcriptome analysis was performed on MM6 cell line using SAGE and the results were compared to the profile of primary human t(9;11) AML cells. 884 transcripts which were alternatively expressed between MM6 cells and primary human t(9;11) cells were identified through statistical analysis (P < 0.05) and 4-fold expression change. of these transcripts, 830 (94%) matched to known genes or EST were classified by functional categories (http://david.abcc. ncifcrf.gov/). The majority of alternatively expressed genes in MM6 were involved in biosynthetic and metabolic processes, but HRAS, a protein that is known to be associated with leukemogenesis, was expressed only in MM6 cells and several other genes involved in Erk1/Erk2 MAPK pathway were also over-expressed in MM6. Therefore, since MM6 cell line has a similar expression profile to primary human t(9;11) AML in general and expresses uniquely a strong Erk1/Erk2 MAPK pathway including HRAS, it can be used as a model for HRAS-positive t(9;11) AML. | - |
dc.publisher | Genetics Soc Japan | - |
dc.title | A comparison of gene expression profiles between primary human AML cells and AML cell line | - |
dc.title.alternative | A comparison of gene expression profiles between primary human AML cells and AML cell line | - |
dc.type | Article | - |
dc.citation.title | Genes & Genetic Systems | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 345 | - |
dc.citation.startPage | 339 | - |
dc.citation.volume | 83 | - |
dc.contributor.affiliatedAuthor | Kyu Tae Chang | - |
dc.contributor.alternativeName | 이진석 | - |
dc.contributor.alternativeName | 황준모 | - |
dc.contributor.alternativeName | 김형수 | - |
dc.contributor.alternativeName | 김성곤 | - |
dc.contributor.alternativeName | 김영훈 | - |
dc.contributor.alternativeName | 박소영 | - |
dc.contributor.alternativeName | 김길수 | - |
dc.contributor.alternativeName | 유재영 | - |
dc.contributor.alternativeName | 장규태 | - |
dc.contributor.alternativeName | 이상규 | - |
dc.identifier.bibliographicCitation | Genes & Genetic Systems, vol. 83, pp. 339-345 | - |
dc.identifier.doi | 10.1266/ggs.83.339 | - |
dc.subject.keyword | AML | - |
dc.subject.keyword | Gene expression | - |
dc.subject.keyword | SAGE | - |
dc.subject.keyword | Translocation | - |
dc.subject.local | AML | - |
dc.subject.local | Gene Expression | - |
dc.subject.local | Gene expression | - |
dc.subject.local | gene expression | - |
dc.subject.local | SAGE | - |
dc.subject.local | Translocation | - |
dc.subject.local | translocation | - |
dc.description.journalClass | Y | - |
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