Egr-1 and serum response factor are involved in growth factors- and serum-mediated induction of E2-EPF UCP expression that regulates the VHL-HIF pathway

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dc.contributor.authorJung Hwa Lim-
dc.contributor.authorCho-Rok Jung-
dc.contributor.authorC H Lee-
dc.contributor.authorDong Soo Im-
dc.date.accessioned2017-04-19T09:11:59Z-
dc.date.available2017-04-19T09:11:59Z-
dc.date.issued2008-
dc.identifier.issn0730-2312-
dc.identifier.uri10.1002/jcb.21914ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8651-
dc.description.abstractE2-EPF ubiquitin carrier protein (UCP) has been shown to be highly expressed in common human cancers and target von Hippel-Lindau (VHL) for proteosomal degradation in cells, thereby stabilizing hypoxia-inducible factor (HIF)-1α. Here, we investigated cellular factors that regulate the expression of UCP gene. Promoter deletion assay identified binding sites for early growth response-1 (Egr-1) and serum response factor (SRF) in the UCP promoter. Hepatocyte or epidermal growth factor (EGF), or phorbol 12-myristate 13-acetate induced UCP expression following early induction of Egr-1 expression in HeLa cells. Serum increased mRNA and protein levels of SRF and UCP in the cell. By electrophoretic mobility shift and chromatin immunoprecipitation assays, sequence-specific DNA-binding of Egr-1 and SRF to the UCP promoter was detected in nuclear extracts from HeLa cells treated with EGF and serum, respectively. Overexpression of Egr-1 or SRF increased UCP expression. RNA interference-mediated depletion of endogenous Egr-1 or SRF impaired EGF- or serum-mediated induction of UCP expression, which was required for cancer cell proliferation. Systemic delivery of EGF into mice also increased UCP expression following early induction of Egr-1 expression in mouse liver. The induced UCP expression by the growth factors or serum increased HIF-1α protein level under non-hypoxic conditions, suggesting that the Egr-1/SRF-UCP-VHL pathway is in part responsible for the increased HIF-1α protein level in vitro and in vivo. Thus, growth factors and serum induce expression of Egr-1 and SRF, respectively, which in turn induces UCP expression that positively regulates cancer cell growth.-
dc.publisherWiley-
dc.titleEgr-1 and serum response factor are involved in growth factors- and serum-mediated induction of E2-EPF UCP expression that regulates the VHL-HIF pathway-
dc.title.alternativeEgr-1 and serum response factor are involved in growth factors- and serum-mediated induction of E2-EPF UCP expression that regulates the VHL-HIF pathway-
dc.typeArticle-
dc.citation.titleJournal of Cellular Biochemistry-
dc.citation.number4-
dc.citation.endPage1127-
dc.citation.startPage1117-
dc.citation.volume105-
dc.contributor.affiliatedAuthorJung Hwa Lim-
dc.contributor.affiliatedAuthorCho-Rok Jung-
dc.contributor.affiliatedAuthorDong Soo Im-
dc.contributor.alternativeName임정화-
dc.contributor.alternativeName정초록-
dc.contributor.alternativeName이찬희-
dc.contributor.alternativeName임동수-
dc.identifier.bibliographicCitationJournal of Cellular Biochemistry, vol. 105, no. 4, pp. 1117-1127-
dc.identifier.doi10.1002/jcb.21914-
dc.subject.keywordE2-EPF UCP-
dc.subject.keywordEgr-1-
dc.subject.keywordGene expression-
dc.subject.keywordGrowth factors-
dc.subject.keywordHIF-1α-
dc.subject.keywordSRF-
dc.subject.keywordVHL-
dc.subject.localE2-EPF UCP-
dc.subject.localEGR1-
dc.subject.localEgr-1-
dc.subject.localEGR-1-
dc.subject.localGene Expression-
dc.subject.localGene expression-
dc.subject.localgene expression-
dc.subject.localgrowth factor-
dc.subject.localGrowth Factor-
dc.subject.localGrowth factors-
dc.subject.localGrowth factor-
dc.subject.localHif1α-
dc.subject.localHIF-1α-
dc.subject.localHIF1α-
dc.subject.localSRF-
dc.subject.localVHL-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
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