Phospholipase A2β mediates light-induced stomatal opening in Arabidopsis

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Title
Phospholipase A2β mediates light-induced stomatal opening in Arabidopsis
Author(s)
J Seo; Hyoung Yool Lee; H Choi; Y Choi; Y Lee; Y W Kim; Stephen Beungtae Ryu; Y Lee
Bibliographic Citation
Journal of Experimental Botany, vol. 59, no. 13, pp. 3587-3594
Publication Year
2008
Abstract
Phospholipase A2 (PLA2) catalyses the hydrolysis of phospholipids into lysophospholipids and free fatty acids. Physiological studies have indicated that PLA2 is involved in stomatal movement. However, genetic evidence of a role of PLA2 in guard cell signalling has not yet been reported. To identify PLA2 gene(s) that is (are) involved in light-induced stomatal opening, stomatal movement was examined in Arabidopsis thaliana plants in which the expression of PLA2 isoforms was reduced or knocked-out. Light-induced stomatal opening in PLA2α knockout plants did not differ from wild-type plants. Plants in which PLA 2β was silenced by RNA interference exhibited delayed light-induced stomatal opening, and this phenotype was reversed by exogenous lysophospholipids, which are products of PLA2. Stomatal opening in transgenic plants that over-expressed PLA2β was faster than wild-type plants. The expression of PLA2β was localized to the endoplasmic reticulum of guard cells, and increased in response to light in the mature leaf. Aristolochic acid, which inhibits light-induced stomatal opening, inhibited the activity of purified PLA2β. Collectively, these results provide evidence that PLA2β is involved in light-induced stomatal opening in Arabidopsis.
Keyword
Guard cellLight signal transductionPhospholipase A2Stomata
ISSN
0022-0957
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/jxb/ern208
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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