ASPMF: A new approach for identifying alternative splicing isoforms using peptide mass fingerprinting

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dc.contributor.authorSeung-Won Lee-
dc.contributor.authorJae Pil Choi-
dc.contributor.authorHyun Jin Kim-
dc.contributor.authorJi-Man Hong-
dc.contributor.authorCheol-Goo Hur-
dc.date.accessioned2017-04-19T09:12:03Z-
dc.date.available2017-04-19T09:12:03Z-
dc.date.issued2008-
dc.identifier.issn0006-291X-
dc.identifier.uri10.1016/j.bbrc.2008.09.115ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8668-
dc.description.abstractAlternative splicing is generally accepted as a mechanism that explains the discrepancy between the number of genes and proteins. We used peptide mass fingerprinting with a theoretical database and scoring method to discover and identify alternative splicing isoforms. Our theoretical database was built using published alternative splicing databases such as ECgene, H-DBAS, and TISA. According to our theoretical database of 190,529 isoforms, 37% of human genes have multiple isoforms. The isoforms produced from a gene partially share common peptide fragments because they have common exons, making it difficult to distinguish isoforms. Therefore, we developed a new method that effectively distinguishes a true isoform among multiple isoforms in a gene. In order to evaluate our algorithm, we made test sets for 4226 protein isoforms extracted from our theoretical database randomly. Consequently, 94% of true isoforms were identified by our scoring algorithm.-
dc.publisherElsevier-
dc.titleASPMF: A new approach for identifying alternative splicing isoforms using peptide mass fingerprinting-
dc.title.alternativeASPMF: A new approach for identifying alternative splicing isoforms using peptide mass fingerprinting-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number1-
dc.citation.endPage256-
dc.citation.startPage253-
dc.citation.volume377-
dc.contributor.affiliatedAuthorSeung-Won Lee-
dc.contributor.affiliatedAuthorJae Pil Choi-
dc.contributor.affiliatedAuthorHyun Jin Kim-
dc.contributor.affiliatedAuthorJi-Man Hong-
dc.contributor.affiliatedAuthorCheol-Goo Hur-
dc.contributor.alternativeName이승원-
dc.contributor.alternativeName최재필-
dc.contributor.alternativeName김현진-
dc.contributor.alternativeName홍지만-
dc.contributor.alternativeName허철구-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 377, no. 1, pp. 253-256-
dc.identifier.doi10.1016/j.bbrc.2008.09.115-
dc.subject.keywordAlternative splicing-
dc.subject.keywordMass spectrometry (MS)-
dc.subject.keywordMS/MS-
dc.subject.keywordPeptide mass fingerprinting (PMF)-
dc.subject.keywordProtein identification-
dc.subject.keywordProtein isoform-
dc.subject.keywordTandem MS-
dc.subject.localAlternative splicing-
dc.subject.localalternative splicing-
dc.subject.localMass spetrometry-
dc.subject.localMass spectrometry (MS)-
dc.subject.localmass spectrometry-
dc.subject.localMass spectrometry-
dc.subject.localmass spectrometry (MS)-
dc.subject.localMS/MS-
dc.subject.localPeptide mass fingerprinting (PMF)-
dc.subject.localpeptide mass fingerprinting-
dc.subject.localProtein identification-
dc.subject.localProtein isoform-
dc.subject.localTandem MS-
dc.description.journalClassY-
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