DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seung-Won Lee | - |
dc.contributor.author | Jae Pil Choi | - |
dc.contributor.author | Hyun Jin Kim | - |
dc.contributor.author | Ji-Man Hong | - |
dc.contributor.author | Cheol-Goo Hur | - |
dc.date.accessioned | 2017-04-19T09:12:03Z | - |
dc.date.available | 2017-04-19T09:12:03Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | 10.1016/j.bbrc.2008.09.115 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8668 | - |
dc.description.abstract | Alternative splicing is generally accepted as a mechanism that explains the discrepancy between the number of genes and proteins. We used peptide mass fingerprinting with a theoretical database and scoring method to discover and identify alternative splicing isoforms. Our theoretical database was built using published alternative splicing databases such as ECgene, H-DBAS, and TISA. According to our theoretical database of 190,529 isoforms, 37% of human genes have multiple isoforms. The isoforms produced from a gene partially share common peptide fragments because they have common exons, making it difficult to distinguish isoforms. Therefore, we developed a new method that effectively distinguishes a true isoform among multiple isoforms in a gene. In order to evaluate our algorithm, we made test sets for 4226 protein isoforms extracted from our theoretical database randomly. Consequently, 94% of true isoforms were identified by our scoring algorithm. | - |
dc.publisher | Elsevier | - |
dc.title | ASPMF: A new approach for identifying alternative splicing isoforms using peptide mass fingerprinting | - |
dc.title.alternative | ASPMF: A new approach for identifying alternative splicing isoforms using peptide mass fingerprinting | - |
dc.type | Article | - |
dc.citation.title | Biochemical and Biophysical Research Communications | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 256 | - |
dc.citation.startPage | 253 | - |
dc.citation.volume | 377 | - |
dc.contributor.affiliatedAuthor | Seung-Won Lee | - |
dc.contributor.affiliatedAuthor | Jae Pil Choi | - |
dc.contributor.affiliatedAuthor | Hyun Jin Kim | - |
dc.contributor.affiliatedAuthor | Ji-Man Hong | - |
dc.contributor.affiliatedAuthor | Cheol-Goo Hur | - |
dc.contributor.alternativeName | 이승원 | - |
dc.contributor.alternativeName | 최재필 | - |
dc.contributor.alternativeName | 김현진 | - |
dc.contributor.alternativeName | 홍지만 | - |
dc.contributor.alternativeName | 허철구 | - |
dc.identifier.bibliographicCitation | Biochemical and Biophysical Research Communications, vol. 377, no. 1, pp. 253-256 | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.09.115 | - |
dc.subject.keyword | Alternative splicing | - |
dc.subject.keyword | Mass spectrometry (MS) | - |
dc.subject.keyword | MS/MS | - |
dc.subject.keyword | Peptide mass fingerprinting (PMF) | - |
dc.subject.keyword | Protein identification | - |
dc.subject.keyword | Protein isoform | - |
dc.subject.keyword | Tandem MS | - |
dc.subject.local | Alternative splicing | - |
dc.subject.local | alternative splicing | - |
dc.subject.local | Mass spetrometry | - |
dc.subject.local | Mass spectrometry (MS) | - |
dc.subject.local | mass spectrometry | - |
dc.subject.local | Mass spectrometry | - |
dc.subject.local | mass spectrometry (MS) | - |
dc.subject.local | MS/MS | - |
dc.subject.local | Peptide mass fingerprinting (PMF) | - |
dc.subject.local | peptide mass fingerprinting | - |
dc.subject.local | Protein identification | - |
dc.subject.local | Protein isoform | - |
dc.subject.local | Tandem MS | - |
dc.description.journalClass | Y | - |
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