Identification of △9-elongation activity from Thraustochytrium aureum by heterologous expression in Pichia pastoris = 효모 Pichia pastoris에서 이종발현에 의한 Thraustochytrium aureum 유래 지방산 축합 효소의 동정

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dc.contributor.authorJ C Lee-
dc.contributor.authorP Anbu-
dc.contributor.authorW H Kim-
dc.contributor.authorM J Noh-
dc.contributor.authorSoo-Jin Lee-
dc.contributor.authorJeong-Woo Seo-
dc.contributor.authorB K Hur-
dc.date.accessioned2017-04-19T09:12:04Z-
dc.date.available2017-04-19T09:12:04Z-
dc.date.issued2008-
dc.identifier.issn1226-8372-
dc.identifier.uri10.1007/s12257-008-0032-5ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8674-
dc.description.abstractThe Δ9-elongase isolated from Thraustochytrium aureum, which contains a high level of polyunsaturated fatty acids (PUFAs), was demonstrated to be associated with the synthesis of C20 PUFAs. The TaELO gene contains a 825 bp ORF that encodes a protein of 274 amino acids that shares a high similarity with other PUFA elongases. The expression of the TaELO gene in Pichia pastoris resulted in the elongation of linoleic acid (LA, C18:2; n-6) and γ-linolenic acid (ALA, C18:3; n-3) to eicosadienoic acid (EDA, C20:2; n-6) and eicosatrienoic acid (ETrA, C20:3; n-3), respectively. The endogenous conversion rate of LA and ALA to EDA and ETrA was 32.68 and 38.57%, respectively. In addition, TaELO was also able to synthesize eicosenoic acid (C20:1; n-9) from oleic acid (OA, C18:1; n-9), even though the conversion level was low (2.81%). Furthermore, TaELO was able to carry out the Δ6-elongation of γ-linolenic acid (GLA, C18:3; n-6) to dihomo-γ-linolenic acid (DGLA, C20:3; n-6) and Δ5-elongation of eicosapentaenoic acid (EPA, C20:5; n-3) to docosapentaenoic acid (DPA, C22:5; n-3). The conversion rate of GLA to DGLA and EPA to DPA were 93 and 28.36%, respectively. The TaELO protein was confirmed to have multifunctional activities, such as Δ9, Δ6, and Δ5-elongations as well as the elongation of monounsaturated fatty acid.-
dc.publisherSpringer-
dc.titleIdentification of △9-elongation activity from Thraustochytrium aureum by heterologous expression in Pichia pastoris = 효모 Pichia pastoris에서 이종발현에 의한 Thraustochytrium aureum 유래 지방산 축합 효소의 동정-
dc.title.alternativeIdentification of △9-elongation activity from Thraustochytrium aureum by heterologous expression in Pichia pastoris-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number5-
dc.citation.endPage532-
dc.citation.startPage524-
dc.citation.volume13-
dc.contributor.affiliatedAuthorSoo-Jin Lee-
dc.contributor.affiliatedAuthorJeong-Woo Seo-
dc.contributor.alternativeName이재철-
dc.contributor.alternativeNameAnbu-
dc.contributor.alternativeName김원호-
dc.contributor.alternativeName노명주-
dc.contributor.alternativeName이수진-
dc.contributor.alternativeName서정우-
dc.contributor.alternativeName허병기-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 13, no. 5, pp. 524-532-
dc.identifier.doi10.1007/s12257-008-0032-5-
dc.subject.keywordeicosadienoic acid-
dc.subject.keywordeicosatrienoic acid-
dc.subject.keywordelongase-
dc.subject.keywordpolyunsaturated fatty acids-
dc.subject.keywordthraustochytrium aureum-
dc.subject.localeicosadienoic acid-
dc.subject.localeicosatrienoic acid-
dc.subject.localelongase-
dc.subject.localElongase-
dc.subject.localPolyunsaturated fatty acids-
dc.subject.localpolyunsaturated fatty acids-
dc.subject.localpolyunsaturated fatty acids (PUFAs)-
dc.subject.localPolyunsaturated fatty acid-
dc.subject.localpolyunsaturated fatty acid-
dc.subject.localThraustochytium aureum-
dc.subject.localthraustochytrium aureum-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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