Cyanobacterial bioreporters for detection of heavy metals, herbicide, and antibiotics = 중금속, 제초제 및 항생제 검출용 남세균 유래 바이로 리포터

Cited 0 time in scopus
Metadata Downloads
Title
Cyanobacterial bioreporters for detection of heavy metals, herbicide, and antibiotics = 중금속, 제초제 및 항생제 검출용 남세균 유래 바이로 리포터
Author(s)
S Y Kim; Won Joong Chung; K H Suh; Jang Ryol Liu; Y I Park
Bibliographic Citation
Journal of Plant Biotechnology, vol. 35, no. 2, pp. 141-145
Publication Year
2008
Abstract
In this study, glucose-inducible intergenic sequences were used to generate bioreporters of the cyanobacterium Synechocystis sp. PCC 6803 that could monitor environmental pollutants. Luciferase genes LuxAB from the marine bacteriumVibrio fischeri under the control of glucose-inducible intergenic seqeucens of eight genes (atpI, ndbA, ctaD1, tkt, pgi, pdh, ppc, and cydA) were successfully expressed in the cyanobacterial transformants, showing 5-25 fold increases in biolumeniscence upon exposure to glucose. In addition, glucose-inducible cyanobacterial bioreporters were very sensitive to various chemicals such as heavy metals (Hg2+, Cu2+, Zn2+), electron transport inhibitors (DCMU, DBMIB, CN-), and antibiotics (chloramphenicol and rifampicin). These glucose-inducible cyanobacterial bioreporters would be useful to develop biosensors for rapid screening of environmental samples.
ISSN
1229-2818
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2008.35.2.141
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.