Cyanobacterial bioreporters for detection of heavy metals, herbicide, and antibiotics = 중금속, 제초제 및 항생제 검출용 남세균 유래 바이로 리포터
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- Title
- Cyanobacterial bioreporters for detection of heavy metals, herbicide, and antibiotics = 중금속, 제초제 및 항생제 검출용 남세균 유래 바이로 리포터
- Author(s)
- S Y Kim; Won Joong Chung; K H Suh; Jang Ryol Liu; Y I Park
- Bibliographic Citation
- Journal of Plant Biotechnology, vol. 35, no. 2, pp. 141-145
- Publication Year
- 2008
- Abstract
- In this study, glucose-inducible intergenic sequences were used to generate bioreporters of the
cyanobacterium Synechocystis sp. PCC 6803 that could monitor environmental pollutants. Luciferase genes
LuxAB from the marine bacteriumVibrio fischeri under the control of glucose-inducible intergenic seqeucens
of eight genes (atpI, ndbA, ctaD1, tkt, pgi, pdh, ppc, and cydA) were successfully expressed in the cyanobacterial
transformants, showing 5-25 fold increases in biolumeniscence upon exposure to glucose. In addition,
glucose-inducible cyanobacterial bioreporters were very sensitive to various chemicals such as heavy
metals (Hg2+, Cu2+, Zn2+), electron transport inhibitors (DCMU, DBMIB, CN-), and antibiotics (chloramphenicol
and rifampicin). These glucose-inducible cyanobacterial bioreporters would be useful to develop biosensors for
rapid screening of environmental samples.
- ISSN
- 1229-2818
- Publisher
- Korea Soc-Assoc-Inst
- DOI
- http://dx.doi.org/10.5010/JPB.2008.35.2.141
- Type
- Article
- Appears in Collections:
- Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
- Files in This Item:
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