Gene expression profiling: Canonical molecular changes and clinicopathological features in sporadic colorectal cancers = 대장암의 유전자 발현 프로화일

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Title
Gene expression profiling: Canonical molecular changes and clinicopathological features in sporadic colorectal cancers = 대장암의 유전자 발현 프로화일
Author(s)
J C Kim; Seon-Young Kim; S A Roh; D H Cho; D D Kim; J H Kim; Yong Sung Kim
Bibliographic Citation
World Journal of Gastroenterology, vol. 14, no. 42, pp. 6662-6672
Publication Year
2008
Abstract
Aim: To investigate alternative or subordinate pathways involved in colorectal tumorigenesis and tumor growth, possibly determining at-risk populations and predicting responses to treatment. Methods: Using microarray gene-expression analysis, we analyzed patterns of gene expression relative to canonical molecular changes and clinicopathological features in 84 sporadic colorectal cancer patients, standardized by tumor location. Subsets of differentially expressed genes were confirmed by real-time reverse-transcript polymerase chain reaction (RT-PCR). Results: The largest number of genes identified as being differentially expressed was by tumor location, and the next largest number by lymphovascular or neural invasion of tumor cells and by mismatch repair (MMR) defects. Amongst biological processes, the immune response was significantly implicated in entire molecular changes observed during colorectal tumorigenesis (P < 0.001). Amongst 47 differentially expressed genes, seven (PISD, NIBP, BAI2, STOML1, MRPL21, MRPL16, and MKKS) were newly found to correlate with tumorigenesis and tumor growth. Most location-associated molecular changes had distinct effects on gene expression, but the effects of the latter were sometimes contradictory. Conclusion: We show that several differentially expressed genes were associated with canonical molecular changes in sporadic colorectal cancers, possibly constituting alternative or subordinate pathways of tumorigenesis. As tumor location was the dominant factor influencing differential gene expression, location-specific analysis may identify location-associated pathways and enhance the accuracy of class prediction.
Keyword
Colorectal adenocarcinomasGene expressionProfilingSporadicTumorigenesis
ISSN
1007-9327
Publisher
Baishideng Publishing Group Inc
DOI
http://dx.doi.org/10.3748/wjg.14.6662
Type
Article
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