Fabrication of CdSe/ZnS quantum-dot-conjugated protein microarrays and nanoarrays = 양자점입자가 부착된 마이크로/나노어레이 제조

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Title
Fabrication of CdSe/ZnS quantum-dot-conjugated protein microarrays and nanoarrays = 양자점입자가 부착된 마이크로/나노어레이 제조
Author(s)
A Gokarna; S K Lee; J S Hwang; Y H Cho; Yong Taik Lim; Bong Hyun Chung; M Lee
Bibliographic Citation
Journal of Korean Physical Society, vol. 53, no. 5, pp. 3047-3050
Publication Year
2008
Abstract
We demonstrate the fabrication and the detection of protein microarrays and nanoarrays, which are tagged by using colloidal CdSe/ZnS quantum dots (QDs), for the purpose of optical readout of those biochips. A microarrayer and a dip-pen nanolithography technique were used for the fabrication of QD-labeled protein microarrays and nanoarrays, respectively. The optical readout of those biochips was conducted by using a microchip scanner and a confocal scanning microscope in the case of the microarrays while an atomic force microscope was employed for characterizing the nanoarrays. We also report a possible way of reducing the 'doughnut phenomenon' in microarrays by varying the spot size from 300 μm to 100 μm, wherein the fluorescence intensity distribution becomes more uniform with decreasing spot diameter.
Keyword
dip-pen nanolithographynanoarraysprotein microarraysquantum dots
ISSN
0374-4884
Publisher
South Korea
DOI
http://dx.doi.org/10.3938/jkps.53.3047
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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