High sensitivity detection of 16s rRNA using peptide nucleic acid probes and a surface plasmon resonance biosensor

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Title
High sensitivity detection of 16s rRNA using peptide nucleic acid probes and a surface plasmon resonance biosensor
Author(s)
Hyou-Arm Joung; Nae-Rym Lee; Seok Ki Lee; Jun Hyoung Ahn; Yong Beom Shin; H S Choi; C S Lee; S Kim; Min-Gon Kim
Bibliographic Citation
Analytica Chimica Acta, vol. 630, no. 2, pp. 168-173
Publication Year
2008
Abstract
A signal enhancing method allowing highly sensitive detection of E. coli 16s rRNA was developed using peptide nucleic acid (PNA) as a capture probe and a surface plasmon resonance (SPR) sensor as a detector. 16s rRNA has been used as a genetic marker for identification of organisms, and can be analyzed directly without PCR amplification due to the relatively high number of copies. PNA has a neutral backbone structure, therefore hybridization with 16s rRNA results in the ionic condition being changed from neutral to negative. A cationic Au nanoparticle was synthesized and used for signal amplification by ionic interaction with 16s rRNA hybridized on the PNA probe-immobilized SPR sensor chip. This method resulted in a detection limit of E. coli rRNA of 58.2 ± 1.37 pg mL-1. Using this analytical method, Staphylococcus aureus was detected without purification of rRNA.
Keyword
16s rRNACationic nanoparticlePeptide nucleic acidSurface plasmon resonance
ISSN
0003-2670
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.aca.2008.10.001
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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