Epiregulin expression by Ets-1 and ERK signaling pathway in Ki-ras-transformed cells

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Title
Epiregulin expression by Ets-1 and ERK signaling pathway in Ki-ras-transformed cells
Author(s)
M C Cho; H S Choi; S Lee; Bo Yeon Kim; M Jung; S N Park; D Y Yoon
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 377, no. 3, pp. 832-837
Publication Year
2008
Abstract
Epiregulin belongs to the epidermal growth factor family, binds to the epidermal growth factor receptor, and its expression is upregulated in various cancer cells, but the regulatory mechanism is unclear. We investigated the regulatory mechanism of epiregulin expression in Ki-ras-transformed cancer cells. In 267B1/Ki-ras cells, the RAF/MEK/ERK pathway was constitutively activated, epiregulin was up-regulated, and the expression and phosphorylation of Ets-1 were augmented. The inhibition of ERK by PD98059 decreased epiregulin and Ets-1 expression and suppressed the growth of 267B1/Ki-ras cells. A chromatin immunoprecipitation assay demonstrated that Ets-1 was bound to human epiregulin promoter, and this binding was abolished by PD98059. Silencing of Ets-1 by RNA interference decreased cellular epiregulin transcript expression. We suggest that the Ki-ras mutation in 267B1 prostate cells constitutively activates the RAF/MEK/ERK pathway and induces the activation of the Ets-1 transcription factor, ultimately leading to the increased expression of epiregulin.
Keyword
EpiregulinEts-1Ki-rasOncogeneProsate cancerRAF/MEK/ERK pathway
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2008.10.053
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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