NDRG2 gene expression in B16F10 melanoma cells restrains melanogenesis via inhibition of Mitf expression

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Title
NDRG2 gene expression in B16F10 melanoma cells restrains melanogenesis via inhibition of Mitf expression
Author(s)
A Kim; Y Yang; M S Lee; Y D Yoo; Hee Gu Lee; J S Lim
Bibliographic Citation
Pigment Cell & Melanoma Research, vol. 21, no. 6, pp. 653-664
Publication Year
2008
Abstract
NDRG2 (N-myc downstream-regulated gene 2) is a candidate tumor suppressor implicated in control of glioblastoma proliferation and dendritic cell differentiation. The microphthalmia-associated transcription factor (Mitf) plays a crucial role in the melanocyte lineage and in melanoma by controlling survival, differentiation, cell cycle entry and exit, and melanoma metastasis. Identifying upstream regulators of Mitf expression, therefore, remains a key issue. In this study, we aimed to assess whether the candidate tumor suppressor NDRG2 can modulate Mitf expression. Here, we show that NDRG2 acts to prevent cAMP and β-catenin-mediated activation of the Mitf promoter, thereby blocking melanogenesis via the downstream Mitf target genes Tyrosinase, Tyrp1 and Dct. The data suggest that NDRG2 impairs melanogenesis by interfering with both the TCF/β-catenin and cAMP/CREB pathways that are known to stimulate Mitf expression in melanocytes and have major implications for the role of NDRG2 in pigmentation and melanoma progression. Taken together, the results not only identify NDRG2 as a novel regulator of pigmentation, but also potentially a key factor in regulating melanoma progression via Mitf.
Keyword
cAMPMelanogenesisMitfNDRG2TCF/β-catenin
ISSN
1755-1471
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1755-148X.2008.00503.x
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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