Isolation and characterization of endophytic actinomycetes from chinese cabbage roots as antagonists to Plasmodiophora brassicae

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Title
Isolation and characterization of endophytic actinomycetes from chinese cabbage roots as antagonists to Plasmodiophora brassicae
Author(s)
S O Lee; G J Choi; Y H Choi; K S Jang; Dong Jin Park; Chang-Jin Kim; J C Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 18, no. 11, pp. 1741-1746
Publication Year
2008
Abstract
This study was conducted to select endophytic actinomycetes as biocontrol agents against Chinese cabbage clubroot caused by Plasmodiophora brassicae. A total of 81 endophytic actinomycetes were isolated from surface-sterilized roots of Chinese cabbage that was grown on paddy field and upland soils collected from various locations in Korea. By using 16S ribosomal DNA (rDNA) gene sequencing, they were classified to 8 actinobacterial genera. The genus Microbispora (67%) was most frequently isolated, followed by Streptomyces (12%) and Micromonospora (11%). Three of the 81 isolates, when inoculated in germinated Chinese cabbage seeds and then transplanted to pots, effectively suppressed the occurrence of a post-inoculated strain of P. brassicae in the pots. They showed control values of 58% for strain A004, 33% for strain A011, and 42% for strain A018. Based on cell wall components, morphological characteristics, and phylogenetic analyses, the three antagonistic isolates were identified as Microbispora rosea subsp. rosea (A004 and A011) and Streptomyces olivochromogenes (A018). Further researches on the field efficacy and action modes of the three actinomycetes are in progress.
Keyword
ClubrootEndophytic actinomyceteMicrobispora rosea subsp. roseaPlasmodiophora brassicaeStreptomyces olivochromogenes
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.0800.108
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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