Exploring the effects of carbon sources on the metabolic capacity for shikimic acid production in Escherichia coli using In silico metabolic predictions = 인실리코대사예측을 통한 다양한 탄소원에 대한 대장균에서 쉬큄산 생산을 위한 대사능력 탐색

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Title
Exploring the effects of carbon sources on the metabolic capacity for shikimic acid production in Escherichia coli using In silico metabolic predictions = 인실리코대사예측을 통한 다양한 탄소원에 대한 대장균에서 쉬큄산 생산을 위한 대사능력 탐색
Author(s)
Jungoh AhnHong-Weon Lee; R Saha; M S Park; Joon Ki Jung; D Y Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 18, no. 11, pp. 1773-1784
Publication Year
2008
Abstract
Effects of various industrially important carbon sources (glucose, sucrose, xylose, gluconate, and glycerol) on shikimic acid (SA) biosynthesis in Escherichia coli were investigated to gain new insight into the metabolic capability for overproducing SA. At the outset, constraints-based flux analysis using the genome-scale in silico model of E. coli was conducted to quantify the theoretical maximum SA yield. The corresponding flux distributions fueled by different carbon sources under investigation were compared with respect to theoretical yield and energy utilization, thereby identifying the indispensable pathways for achieving optimal SA production on each carbon source. Subsequently, a shikimate-kinase-deficient E. coli mutant was developed by blocking the aromatic amino acid pathway, and the production of SA on various carbon sources was experimentally examined during 51 batch culture. As a result, the highest production rate, 1.92 mmol SA/h, was obtained when glucose was utilized as a carbon source, whereas the efficient SA production from glycerol was obtained with the highest yield, 0.21 mol SA formed per mol carbon atom of carbon source consumed. The current strain can be further improved to satisfy the theoretically achievable SA production that was predicted by in silico analysis.
Keyword
Carbon sourcesConstraints-based flux analysisEscherichia coliGenome-scale in silico modelShikimic acid production
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.0700.705
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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