Negative feedback regulation of Aurora-a via phosphorylation of Fas-associated factor-1

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dc.contributor.authorM S Jang-
dc.contributor.authorJ W Sul-
dc.contributor.authorB J Choi-
dc.contributor.authorS J Lee-
dc.contributor.authorJ H Suh-
dc.contributor.authorNam-Soon Kim-
dc.contributor.authorW H Kim-
dc.contributor.authorD S Lim-
dc.contributor.authorC W Lee-
dc.contributor.authorE Kim-
dc.date.accessioned2017-04-19T09:12:24Z-
dc.date.available2017-04-19T09:12:24Z-
dc.date.issued2008-
dc.identifier.issn00219258-
dc.identifier.uri10.1074/jbc.M804199200ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8737-
dc.description.abstractThis study reports that Aurora-A (Aur-A) phosphorylates Fas-associated factor-1 (FAF1) at Ser-289 and Ser-291. Forced expression of a FAF1 mutant mimicking phosphorylation at Ser-289 and Ser-291 (FAF1 DD), but not phosphorylation-deficient FAF1 (FAF1 AA), reduced Aur-A expression. However, transfection of FAF1 DD failed to reduce Aur-A expression in the presence of MG132 and MG115, indicating that this decrease is proteasome-mediated. Additionally, transfection of FAF1 DD suppressed the expression of Aur-A in ts20-BALB cells lacking E1 ubiquitin (Ub) activating enzyme activity at restrictive temperatures and also reduced the expression of Aur-A S51D, a mutant resistant to Ub-dependent degradation. Our data indicate that phosphorylated FAF1 mediates the ubiquitin-independent, proteasome-dependent degradation of Aur-A. Overexpression of FAF1 DD blocked Aur-A-induced centrosome amplification and accumulated cells in G2/M phase, representing cellular phenotypes consistent with the anticipated loss of Aur-A. Collectively, our findings support the negative feedback regulation of Aur-A via phosphorylation of the death-promoting protein, FAF1, and disclose the presence of molecular cross-talk between constituents of the cell cycle and cell death machinery.-
dc.publisherAmer Soc Biochemistry Molecular Biology Inc-
dc.titleNegative feedback regulation of Aurora-a via phosphorylation of Fas-associated factor-1-
dc.title.alternativeNegative feedback regulation of Aurora-a via phosphorylation of Fas-associated factor-1-
dc.typeArticle-
dc.citation.titleJournal of Biological Chemistry-
dc.citation.number47-
dc.citation.endPage32351-
dc.citation.startPage32344-
dc.citation.volume283-
dc.contributor.affiliatedAuthorNam-Soon Kim-
dc.contributor.alternativeName장문선-
dc.contributor.alternativeName설지원-
dc.contributor.alternativeName최병중-
dc.contributor.alternativeName이수진-
dc.contributor.alternativeName서지희-
dc.contributor.alternativeName김남순-
dc.contributor.alternativeName김우호-
dc.contributor.alternativeName임대식-
dc.contributor.alternativeName이창우-
dc.contributor.alternativeName김은희-
dc.identifier.bibliographicCitationJournal of Biological Chemistry, vol. 283, no. 47, pp. 32344-32351-
dc.identifier.doi10.1074/jbc.M804199200-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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