High frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi)

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dc.contributor.authorOh Myung Jin-
dc.contributor.authorH R Na-
dc.contributor.authorH K Choi-
dc.contributor.authorJang Ryol Liu-
dc.contributor.authorSuk Weon Kim-
dc.date.accessioned2017-04-19T09:12:25Z-
dc.date.available2017-04-19T09:12:25Z-
dc.date.issued2008-
dc.identifier.issn1863-5466-
dc.identifier.uri10.1007/s11816-008-0047-6ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8740-
dc.description.abstractAn improved protocol for high frequency plant regeneration via somatic embryogenesis from zygotic embryo-derived cell suspension cultures of watershield (Brasenia schreberi) was developed. Zygotic embryos formed pale-yellow globular structures and white friable callus at a frequency of 80% when cultured on halfstrength MS medium supplemented with 0.3 mg l-1 2,4-D. However, the frequency of formation of pale-yellow globular structures and white friable callus decreased slightly with increasing concentrations of 2,4-D up to 3 mg l-1, where the frequency reached *50% of the control. Cell suspension cultures from zygotic embryoderived white friable callus were established using halfstrength MS medium supplemented with 0.3 mg l-1 2,4-D. Upon plating of cell aggregates on half-strength MS basal medium, approximately 8.3% gave rise to somatic embryos and developed into plantlets. However, the frequency of plantlet development from cell aggregates was sharply increased (by up to 55%) when activated charcoal and zeatin were applied. Regenerated plantlets were successfully transplanted to potting soil and grown to normal plants in a growth chamber. The distinctive feature of this study is the establishment of a high frequency plant regeneration system via somatic embryogenesis from zygotic embryo-derived cell suspension cultures of watershield, which has not been previously reported. The protocol for plant regeneration of watershield through somatic embryogenesis could be useful for the mass propagation and transformation of selected elite lines.-
dc.publisherSpringer-
dc.titleHigh frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi)-
dc.title.alternativeHigh frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi)-
dc.typeArticle-
dc.citation.titlePlant Biotechnology Reports-
dc.citation.number1-
dc.citation.endPage92-
dc.citation.startPage87-
dc.citation.volume2-
dc.contributor.affiliatedAuthorOh Myung Jin-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.contributor.affiliatedAuthorSuk Weon Kim-
dc.contributor.alternativeName오명진-
dc.contributor.alternativeName나혜련-
dc.contributor.alternativeName최홍근-
dc.contributor.alternativeName유장렬-
dc.contributor.alternativeName김석원-
dc.identifier.bibliographicCitationPlant Biotechnology Reports, vol. 2, no. 1, pp. 87-92-
dc.identifier.doi10.1007/s11816-008-0047-6-
dc.subject.keywordbrasenia schreberi-
dc.subject.keywordplant regeneration-
dc.subject.keywordsomatic embryogenesis-
dc.subject.keywordzygotic embryo culture-
dc.subject.localbrasenia schreberi-
dc.subject.localBrasenia schreberi-
dc.subject.localplant regeneration-
dc.subject.localPlant regeneration-
dc.subject.localsomatic embryogenisis-
dc.subject.localsomatic embryogenesis-
dc.subject.localSomatic embryogenesis-
dc.subject.localzygotic embryo culture-
dc.subject.localZygotic embryo culture-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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