Analysis and characterization of hepatitis B vaccine particles synthesized from Hansenula polymorpha

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Title
Analysis and characterization of hepatitis B vaccine particles synthesized from Hansenula polymorpha
Author(s)
H S Seo; J S Park; K Y Han; K D Bae; S J Ahn; Hyun Ah Kang; J Lee
Bibliographic Citation
Vaccine, vol. 26, no. 33, pp. 4138-4144
Publication Year
2008
Abstract
The biochemical and physical properties of hepatitis B virus (HBV) small surface antigen (S-HBVsAg) from Berna Biotech Korea Corp. were systematically analyzed and characterized. Through various electrophoresis and immunoblotting assay of S-HBVsAg and its proteolytic products, it was confirmed that the S-HBVsAg vaccine particles are present in the form of covalent multimers that are assembled via strong intermolecular disulfide bonds. The S-HBVsAg particles contain no N-glycosylation moiety but some O-glycosidically linked mannoses. Evidently from N-terminus sequencing of both monomers and dimers that are formed by complete and partial reduction, respectively, of the S-HBVsAg particles under reducing SDS-PAGE condition, it is evident that each polypeptide within S-HBVsAg particles has authentic sequence of N-terminus. Denaturation plot shows that the S-HBVsAg vaccine particles were extremely stable especially in the solution with high acidity. This stability property of S-HBVsAg vaccine particles could provide very useful information for the optimization of the downstream process of recombinant S-HBVsAg particles synthesized from yeast cultures.
Keyword
Covalent multimerGlycosylationHepatitis B surface antigenStabilityYeast
ISSN
0264-410X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.vaccine.2008.05.070
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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