Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system

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Title
Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system
Author(s)
Yong Taik Lim; Mi Young Jo; Jeong Min Lee; Sang Jeon Chung; Bong Hyun Chung
Bibliographic Citation
Biomaterials, vol. 30, no. 6, pp. 1197-1204
Publication Year
2009
Abstract
Cellular internalization of functional nanoparticles that have optical and magnetic properties is very important in the cellular imaging and manipulation of specifically targeted biomolecules. In this study, a robust method to deliver functional nanoparticles and targeting antibodies into cells was suggested. The engineered protein G system, which contains an affinity tag and a cell penetration peptide in the N- and C-terminals, respectively, can capture surface-modified nanoparticles and antibodies without chemical reaction, and then non-invasively deliver them into the cells. Finally, gold-coated iron oxide nanoparticle/engineered protein G hybrid systems were successfully employed as multifunctional cargo systems for the targeting, imaging, and manipulation of mitochondria.
Keyword
cell imagingengineered proteingoldintracellular deliverynanoparticlesurface modification
ISSN
0142-9612
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biomaterials.2008.11.007
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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