Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system
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- Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system
- Yong Taik Lim; Mi Young Jo; Jeong Min Lee; Sang Jeon Chung; Bong Hyun Chung
- Bibliographic Citation
- Biomaterials, vol. 30, no. 6, pp. 1197-1204
- Publication Year
- Cellular internalization of functional nanoparticles that have optical and magnetic properties is very important in the cellular imaging and manipulation of specifically targeted biomolecules. In this study, a robust method to deliver functional nanoparticles and targeting antibodies into cells was suggested. The engineered protein G system, which contains an affinity tag and a cell penetration peptide in the N- and C-terminals, respectively, can capture surface-modified nanoparticles and antibodies without chemical reaction, and then non-invasively deliver them into the cells. Finally, gold-coated iron oxide nanoparticle/engineered protein G hybrid systems were successfully employed as multifunctional cargo systems for the targeting, imaging, and manipulation of mitochondria.
- cell imagingengineered proteingoldintracellular deliverynanoparticlesurface modification
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- 1. Journal Articles > Journal Articles
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