Abnormal gene expression in extraembryonic tissue from cloned porcine embryos

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Title
Abnormal gene expression in extraembryonic tissue from cloned porcine embryos
Author(s)
Jung Il Chea; Kyu-Sun Lee; D J Kim; Y M Han; D S Lee; Kyung Kwang Lee; Deog Bon Koo
Bibliographic Citation
Theriogenology, vol. 71, no. 2, pp. 323-333
Publication Year
2009
Abstract
The birth rate of cloned animals following somatic cell nuclear transfer (SCNT) is very low and the surviving animals have various developmental defects. We compared the morphology and transcriptional profile of extraembryonic tissue from three 26-d old SCNT pig fetuses with that from control fetuses. Transcriptional profiling using long-oligonucleotide microarray technology revealed 34 genes that were differentially expressed between the three groups. The differential expression of several genes involved in translational regulation was confirmed by real-time quantitative PCR and Western blot analysis. Interestingly, the expression of a translational inhibition-related gene encoding a eukaryotic translation initiation factor 4E-binding protein was significantly elevated in the SCNT samples. We concluded that the low birth rate of cloned animals could be related to abnormal expression of translational regulators in extraembryonic tissue during early pregnancy.
Keyword
Cloned embryoExtraembryonic tissueSomatic cell nuclear transferTranscriptional profiling
ISSN
0093-691X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.theriogenology.2008.07.025
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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