Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer

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Title
Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer
Author(s)
W H Lee; K W Cho; S Y Park; Kee Sun Shin; D S Lee; S K Hwang; S J Seo; J M Kim; S Y Ghim; B H Song; S H Lee; J G Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 18, no. 12, pp. 1869-1873
Publication Year
2008
Abstract
An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4o degrees C after cell proliferation at 20 degrees C. The isolated bacterium was categorized as Gramnegative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.
Keyword
Eicosapentaenoic acidShewanella sp. KMG42716S rDNAphylogenetic tree
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.0800.012
Type
Article
Appears in Collections:
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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